Cytokine responses in mice infected withEmphasis

Authors: Yang Kyu Choi Byung Il Yoon Young Suk Won Chul Ho Lee Byung Hwa Hyun Hyoung Chin Kim Goo Taeg Oh Dae Yong Kim

Publish Date: 2003/07/29

Volume: 91, Issue: 2, Pages: 87-93

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Abstract

FVB and BALB/c mice show different morbidity development of Clonorchis sinensis and pathological changes following C sinensis infection FVB mice are susceptible and BALB/c mice are relatively more resistant to C sinensis infection To investigate the relationship between cytokine reaction and susceptibility to C sinensis infection in FVB and BALB/c mice we described both the patterns and kinetics of Th1 cytokines and Th2 cytokines in spleen cell culture Interleukin IL4 and IL10 cytokine production in the culture supernatants of the concanavalinAstimulated spleen cells increased at 2–3 weeks postinfection in both strains IL5 production increased between 2 and 5 weeks postinfection in both strains and reached a peak level at 2 weeks postinfection in BALB/c mice and 4 weeks postinfection in FVB mice In contrast gamma interferon IFNγ production decreased between 2 and 4 weeks in both strains IL2 production increased slightly in BALB/c mice following infection but was unchanged in FVB mice IL4 production over preinfection levels was significantly higher in FVB mice whereas IFNγ IL2 and IL10 production were significantly higher in BALB/c mice The levels of serum immunoglobulin E IgE and blood eosinophils in both mouse strains significantly increased between 3 and 6 weeks postinfection Serum IgE levels were significantly higher in FVB mice than in BALB/c mice The results of this study suggest that susceptibility to C sinensis infection is associated with Th2 cytokine production especially IL4 which is predominant in relatively susceptible FVB miceWe thank Dr Todd Reinhart Department of Infectious Diseases and Microbiology University of Pittsburgh USA for helpful comments on the manuscript This work was supported by grants KBS0120313 and BDM0300211 from the Ministry of Science and Technology of Korea This work was also partially supported by the Brain Korea 21 Project

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