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Title of Journal: Parasitol Res

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Abbravation: Parasitology Research

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Springer-Verlag

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DOI

10.1002/ange.201404020

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1432-1955

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Emphasis Type="Italic"Acanthamoeba castellanii/

Authors: Bibiana ChávezMunguía Lizbeth SalazarVillatoro Anel LagunesGuillén Maritza OmañaMolina Martha EspinosaCantellano Adolfo MartínezPalomo
Publish Date: 2013/01/15
Volume: 112, Issue: 3, Pages: 1125-1130
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Abstract

During Acanthamoeba castellanii trophozoite–cysts differentiation four morphological stages were identified by scanning electron microscopy trophozoite precyst immature cysts and mature cysts Fluorescence microscopy reveals the presence of small cumulus of actin in the cytoplasm of precysts after treatment with rhodamine phalloidin By the contrary in mature cysts fluorescence was not observed However when excystation was induced large fluorescent patches were present By transmission electron microscopy encysting amebas showed small cytoplasmic vesicles containing fibrillar material surrounded by a narrow area of thin fibrils Similar appearance was observed in pseudopods and phagocytic invaginations In addition large aggregates of rodshape elements similar to the chromatoid bodies described in other amebas were present in the cytoplasm These cysts presented large areas with orange fluorescence after treatment with acridine orange

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