Systemic and local mucosal immune responses induce

Authors: Hongling Qu Yanquan Xu Hengchang Sun Jinsi Lin Jinyun Yu Zeli Tang Jiqing Shen Chi Liang Shan Li Wenjun Chen Xuerong Li Zhongdao Wu Yan Huang Xinbing Yu

Publish Date: 2014/06/07

Volume: 113, Issue: 8, Pages: 3095-3103

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Abstract

Human clonorchiasis caused by Clonorchis sinensis C sinensis has been increasingly prevalent in recent years so that an effective measure is essential and urgent to control the infectious disease Oral delivery of antigens from C sinensis may be an important approach to effectively induce both systemic and local immune responses to antiinfection of the parasite In the current study we used Bacillus subtilis B subtilis spores as a delivery vehicle to introduce leucine aminopeptidase 2 of C sinensis CsLAP2 an excretory/secretory antigen with high immunogenicity expressing on their surface SDSPAGE western blotting and flow cytometry indicated that CsLAP2 was successfully expressed on the surface of B subtilis spores CotCCsLAP2 spores BALB/c mice were treated with spores intragastrically On day 31 after the treatment we found that mice intragastrically treated with CotCCsLAP2 spores exhibited higher IgG IgG1 IgG2a and IgA level in sera as well as higher sIgA level in bile and intestinal lavage fluid compared to mice orally administrated with spores not expressing CsLAP2 CotC spores and naïve mice The peak titer of IgG/IgA presented on day 31/49 after oral administration IgG1 level was lower than IgG2a in group administrated with CotCCsLAP2 spores sIgAsecreting cells were obviously observed in intestinal epithelium of mice orally treated with CotCCsLAP2 spores After incubated with CotCCsLAP2 the levels of IFNγ IL6 IL10 IL17A and TNF significantly increased in the supernatant of splenocytes isolated from mice orally treated with CotCCsLAP2 spores while there was no statistically significant difference of IL4 level representing Th2 response among the groups Our study demonstrated that oral administration of CsLAP2 delivered by B subtilis spore elicited obvious systemic and local mucosal immunity Secretory IgA and Th1Th17 cellular immunity might involved in mechanisms of the immune responseThis work was supported by the National Key Basic Research and Development Project of China 973 project No 2010CB530000 National Natural Science Foundation of China No81101270 and No 81171602 the National S T Major Program 2012ZX10004220 Fundamental Research Funds for the Central Universities of China No 3164015 and No 3161036 and Innovative Research Teams Project of South Wisdom Valley Shunde Guangdong province 2013CXTD03

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