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Title of Journal: Parasitol Res

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Abbravation: Parasitology Research

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Springer-Verlag

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1432-1955

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Rapid diagnosis of Emphasis Type="Italic"Tetraca

Authors: Mansour ElMatbouli Hatem Soliman
Publish Date: 2005/05/14
Volume: 96, Issue: 5, Pages: 277-284
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Abstract

A new molecular diagnostic assay was developed for detection of Tetracapsuloides bryosalmonae the causative agent of proliferative kidney disease PKD in salmonid fish using a loopmediated isothermal amplification method LAMP The PKDLAMP assay amplifies the T bryosalmonae DNA extracted from infected kidney under constant temperature of 65°C within 1 h The required equipment for DNA amplification is only a water bath The amplification products were detected visually by using SYBR green I dye which turns green in the presence of amplified products and remains orange in its absence and by electrophoresis without any difference in the sensitivity of both methods The developed PKDLAMP assay demonstrated an exceptionally higher sensitivity than the conventional PCR PKDLAMP assay was found to be 100fold more sensitive than the PCR assay The developed assay is simple rapid costeffective specific and highly sensitive The assay is also characterized by its field applicability as it does not require the use of sophisticated equipment or skilled personnel

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