Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Parasitol Res

Search In Journal Title:

Abbravation: Parasitology Research

Search In Journal Abbravation:

Publisher

Springer-Verlag

Search In Publisher:

DOI

10.1002/ca.20164

Search In DOI:

ISSN

1432-1955

Search In ISSN:
Search In Title Of Papers:

Multiplex PCR on single unembryonated Emphasis Ty

Authors: Josefine Carlsgart Allan Roepstorff Peter Nejsum
Publish Date: 2008/12/19
Volume: 104, Issue: 4, Pages: 939-
PDF Link

Abstract

A sensitive and inexpensive method for DNA isolation and amplification by polymerase chain reaction PCR from single unembryonated Ascaris sp eggs is described The resistant shell of single eggs was crushed mechanically and PCR applied to the crude egg contents without any further purification steps The ITS1 region of the rDNA and three regions of the mtDNA could be successfully amplified Using two primer sets it was possible to amplify the rDNA and mtDNA simultaneously in one single reaction The ability to perform PCR on single unembryonated eggs may result in better and more precise species identification of eggs recovered from faecal material environmental samples and possibly archaeological samples In addition single egg PCR makes it possible to perform population genetic studies without having to recover adult worms by deworming or autopsy

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Angiostrongylus vasorum (Baillet, 1866) Kamensky, 1905: emergence of third-stage larvae from infected Biomphalaria glabrata snails
  2. Effect of Nigella sativa oil on experimental toxoplasmosis
  3. Erratum to: Development of amultiplex PCR for identification of Dictyocaulus lungworms in domestic and wild ruminants
  4. Detection of Anaplasma marginale in Hyalomma asiaticum ticks by PCR assay
  5. Tetracapsuloides bryosalmonae infection affects the expression of genes involved in cellular signal transduction and iron metabolism in the kidney of the brown trout Salmo trutta
  6. Comparative pharmacokinetics of ivermectin alone and a novel formulation of ivermectin and rafoxanide in calves and sheep
  7. The siRNA-mediated silencing of Trichinella spiralis nudix hydrolase results in reduction of larval infectivity
  8. Expression analysis of Entamoeba invadens profilins in encystation and excystation
  9. Evaluation of the therapeutic and protective effects of ivermectin and permethrin in controlling of wound myiasis infestation in sheep
  10. Effect of mefloquine administered orally at single, multiple, or combined with artemether, artesunate, or praziquantel in treatment of mice infected with Schistosoma japonicum
  11. PCR diagnosis of Pneumocystis carinii on sputum and bronchoalveolar lavage samples in immuno-compromised patients
  12. Curcumin overcomes the inhibitory effect of nitric oxide on Leishmania
  13. Widespread trypanosome infections in a population of eastern hellbenders ( Cryptobranchus alleganiensis alleganiensis ) in Virginia, USA
  14. In vitro evaluation of the amebicidal activity of Pterocaulon polystachyum (Asteraceae) against trophozoites of Acanthamoeba castellanii
  15. In vitro evaluation of the amebicidal activity of Pterocaulon polystachyum (Asteraceae) against trophozoites of Acanthamoeba castellanii
  16. Dietary selenium affects intestinal development of Eimeria papillata in mice
  17. Malaria associated apoptosis is not significantly correlated with either parasitemia or the number of previous malaria attacks
  18. Larvicidal and ovicidal activity of Cassia fistula Linn. leaf extract against filarial and malarial vector mosquitoes
  19. Immunomodulatory effect of R848 on cytokine production associated with Schistosoma mansoni infection
  20. Genotyping of Cryptosporidium isolates from human clinical cases in Poland
  21. The life cycle of Thelohanellus kitauei (Myxozoa: Myxosporea) infecting common carp ( Cyprinus carpio ) involves aurantiactinomyxon in Branchiura sowerbyi
  22. Caveolins and flotillin-2 are present in the blood stages of Plasmodium vivax
  23. The influence of zinc in mice on infection with Angiostrongylus cantonensis
  24. Relationships between local and regional species richness in flea communities of small mammalian hosts: saturation and spatial scale
  25. Tegumental surface changes in adult Fasciola hepatica following treatment in vitro and in vivo with an experimental fasciolicide
  26. Inoculation of salivary gland extracts obtained from female of Rhipicephalus sanguineus (Latreille, 1806) (Acari, Ixodidae) with 2, 4, and 6 days of feeding in rabbit: I—histopathology of the feeding lesion
  27. The efficacy of therapy with albendazole in mice with parasitic meningitis caused by Angiostrongylus cantonensis
  28. The complete mitochondrial genome of the rodent intra-arterial nematodes Angiostrongylus cantonensis and Angiostrongylus costaricensis
  29. Ticks status in Central Asia with a special emphasis on Uzbekistan
  30. Chemotherapy in alveolar echinococcosis of multi-organs: what’s the role?
  31. Ultrastructure of the spermatozoon of Taeniarhynchus saginatus (syn. Taenia saginata ) (Goeze, 1782) Weinland, 1858 (Cestoda, Taeniidae) an intestinal parasite of human
  32. Interaction of BOP1, a protein for ribosome biogenesis, with EB1 in Giardia lamblia
  33. Prevalence and genotyping of Giardia in husbandry systems in Taiwan
  34. Acanthamoeba castellanii cysts: new ultrastructural findings
  35. The stomal morphology of parasitic females of Strongyloides spp. by scanning electron microscopy
  36. Spermatological characters of the spathebothriidean tapeworm Didymobothrium rudolphii (Monticelli, 1890)
  37. Differential expressions of MHC-DQB1 mRNA in Chinese merino sheep infected with Echinococosus granuclosus
  38. Effects of temperature, salinity, and water level on the emergence of marine cercariae
  39. The intestinal nematode Trichuris arvicolae affects the fecundity of its host, the common vole Microtus arvalis
  40. Molecular diversity of avian schistosomes in Danish freshwater snails
  41. Sarcophaga ( Liosarcophaga ) tibialis Macquart 1851 (Diptera: Sarcophagidae): micromorphology of preimaginal stages of a fly of medical and veterinary interest
  42. Fasciola hepatica : disruption of spermatogenesis by the fasciolicide compound alpha
  43. Extracellular release of the surface metalloprotease, gp63, from Leishmania and insect trypanosomatids
  44. Survey of Trichinella infection from domestic pigs in the historical endemic areas of Henan province, central China
  45. Prevalence and intensity of haemogregarinid blood parasites in a population of the Iberian rock lizard, Lacerta monticola
  46. Biosynthesis, characterization, and acute toxicity of Berberis tinctoria -fabricated silver nanoparticles against the Asian tiger mosquito, Aedes albopictus, and the mosquito predators Toxorhynchites splendens and Mesocyclops thermocyclopoides
  47. Anthelminthic efficacy of banana crop residues on gastrointestinal nematodes of sheep: in vitro and in vivo tests
  48. Transcriptome profilings of female Schistosoma japonicum reveal significant differential expression of genes after pairing
  49. Trichobilharzia spp. in natural conditions in Annecy Lake, France
  50. Metazoan ectoparasites of Atlantic mackerel, Scomber scombrus (Teleostei: Scombridae): macro- and microhabitat distribution
  51. In vitro evaluation of trypanocidal activity in plants used in Argentine traditional medicine
  52. Prokineticin receptor identified by phage display is an entry receptor for Trypanosoma cruzi into mammalian cells
  53. Leishmania antimony resistance: what we know what we can learn from the field
  54. In vitro studies on the sexual maturation of the bovine lungworm Dictyocaulus viviparus during the development of preadult larvae to adult worms
  55. Besnoitia besnoiti tachyzoites induce monocyte extracellular trap formation
  56. Partial protective of chickens against Eimeria tenella challenge with recombinant EtMIC-1 antigen
  57. Neem seed extract shampoo, Wash Away Louse®, an effective plant agent against Sarcoptes scabiei mites infesting dogs in Egypt
  58. Morphological effects of neem ( Azadirachta indica A. Juss) seed oil with known azadirachtin concentrations on the oocytes of semi-engorged Rhipicephalus sanguineus ticks (Acari: Ixodidae)
  59. Rapid diagnosis of Tetracapsuloides bryosalmonae , the causative agent of proliferative kidney disease (PKD) in salmonid fish by a novel DNA amplification method, loop-mediated isothermal amplification (LAMP)
  60. West Nile virus in mosquitoes in Greece
  61. First record of myiasis in Aplastodiscus arildae (Anura: Hylidae) by Notochaeta bufonivora (Diptera: Sarcophagidae) in the Neotropical area
  62. Muscle attachment site (MAS) patterns for species determination in European species of Lucilia (Diptera: Calliphoridae)
  63. Excretory/secretory products of sheep abomasal nematode parasites cause vacuolation and increased neutral red uptake by HeLa cells
  64. Comparison of preparation techniques of mixed samples (fungi–helminth eggs) for scanning electron microscopy by critical point drying
  65. Sarcocystis spp. in birds of the order Anseriformes
  66. Larvicidal activity of silver nanoparticles synthesized using Pergularia daemia plant latex against Aedes aegypti and Anopheles stephensi and nontarget fish Poecillia reticulata
  67. Associations between fish reproductive cycle and the dynamics of metazoan parasite infection
  68. Gastrointestinal parasite fauna of Emperor Penguins ( Aptenodytes forsteri ) at the Atka Bay, Antarctica
  69. Marked for life: muscle attachment site patterns in blowfly larvae are constant throughout development
  70. Intra-cystic concentrations of albendazole-sulphoxide in human cystic echinococcosis: a systematic review and analysis of individual patient data
  71. Investigation of Neospora caninum , Hammondia sp., and Toxoplasma gondii in tissues from slaughtered beef cattle in Bahia, Brazil
  72. Silencing of xylose isomerase and cellulose synthase by siRNA inhibits encystation in Acanthamoeba castellanii
  73. Ovicidal, larvicidal and adulticidal properties of Asparagus racemosus (Willd.) (Family: Asparagaceae) root extracts against filariasis ( Culex quinquefasciatus ), dengue ( Aedes aegypti ) and malaria ( Anopheles stephensi ) vector mosquitoes (Diptera: Culicidae)
  74. Contamination of the environment by strongylid (Nematoda: Strongylidae) infective larvae at horse farms of various types in Ukraine
  75. Biochemical studies on glutathione S-transferase from the bovine filarial worm Setaria digitata
  76. The new national integrated strategy emphasizing infection sources control for schistosomiasis control in China has made remarkable achievements
  77. Systemic and local mucosal immune responses induced by orally delivered Bacillus subtilis spore expressing leucine aminopeptidase 2 of Clonorchis sinensis
  78. In vitro anthelmintic activity of the essential oils of Zanthoxylum zanthoxyloides and Newbouldia laevis against Strongyloides ratti
  79. Development of a PCR technique specific for Demodex injai in biological specimens
  80. Excreted/secreted glycoproteins of G. intestinalis play an essential role in the antibody response
  81. Reproductive anatomy and fecundity estimation of the haematophagous ectoparasite Carnus hemapterus
  82. First report of two Balantidium species from the Chinese giant salamander, Andrias davidianus : Balantidium sinensis Nie 1935 and Balantidium andianusis n. sp.
  83. Induction of proinflammatory cytokines and nitric oxide by Trypanosoma cruzi in renal cells
  84. Induction of proinflammatory cytokines and nitric oxide by Trypanosoma cruzi in renal cells
  85. Proteomic identification of potential Clonorchis sinensis excretory/secretory products capable of binding and activating human hepatic stellate cells
  86. Epidemiological investigation of Clonorchis sinensis infection in freshwater fishes in the Pearl River Delta
  87. Synergy between rhinacanthins from Rhinacanthus nasutus in inhibition against mosquito cytochrome P450 enzymes
  88. PCR identification of Culicoides dewulfi midges (Diptera: Ceratopogonidae), potential vectors of bluetongue in Germany
  89. Two extra chromosomal genomes of Leucocytozoon caulleryi ; complete nucleotide sequences of the mitochondrial genome and existence of the apicoplast genome
  90. Macrophage migration inhibitory factor homolog from Plasmodium yoelii modulates monocyte recruitment and activation in spleen during infection
  91. Infestation status of gnathiid isopod juveniles parasitic on Dusky grouper ( Epinephelus marginatus ) from the northeast Mediterranean Sea
  92. A stochastic model of Echinococcus multilocularis transmission in Hokkaido, Japan, focusing on the infection process
  93. Glycogen as a carbohydrate energy reserve in trophozoites of Giardia lamblia
  94. PCR-RFLP analysis: a promising technique for host species identification of blood meals from tsetse flies (Diptera: Glossinidae)
  95. Cytokine responses in mice infected with Clonorchis sinensis
  96. Helminth community structure in the Argentinean bufonid Melanophryniscus klappenbachi : importance of habitat use and season
  97. Gene migration for re-emerging amebiasis in Iran’s northwest–Iraq borders: a microevolutionary scale for reflecting epidemiological drift of Entamoeba histolytica metapopulations
  98. Morphological and genetic characteristics of the anisakid nematode Raphidascaris acus from the southwest Caspian Sea: evidence for the existence of sibling species within a species complex
  99. Heterogeneity of class I and class II MHC sequences in Schistosoma japonicum from different endemic regions in mainland China
  100. Large-scale survey of the prevalence of Eimeria infections in domestic rabbits in China
  101. Oviposition deterrent activity of three mosquito repellents diethyl phenyl acetamide (DEPA), diethyl m toluamide (DEET), and diethyl benzamide (DEB) on Aedes aegypti , Aedes albopictus , and Culex quinquefasciatus
  102. Cryptosporidium and Giardia spp. infections in humans, animals and the environment in Poland
  103. Expression of erythropoietic cytokines in α-tocopherol transfer protein knockout mice with murine malaria infection
  104. Guideline adherence for the treatment of advanced schistosomiasis japonica in Hubei, China
  105. Colonization of Aedes aegypti midgut by the endosymbiont-bearing trypanosomatid Blastocrithidia culicis
  106. Sarcoptes mite from collection to DNA extraction: the lost realm of the neglected parasite
  107. Testing the use of a citronella-based repellent as an effective method to reduce the prevalence and abundance of biting flies in avian nests
  108. In vivo anthelmintic activity of Carex baccans and its active principle resveratrol against Hymenolepis diminuta
  109. Eimeria biarmicus sp.n. (Apicomplexa: Eimeriidae) infecting falcons from the genus Falco in Saudi Arabia
  110. A nested PCR assay exhibits enhanced sensitivity for detection of Theileria parva infections in bovine blood samples from carrier animals
  111. The monocyte locomotion inhibitory factor produced by Entamoeba histolytica inhibits induced nitric oxide production in human leukocytes
  112. Rhipidocotyle fennica (Digenea: Bucephalidae) from Anodonta anatina and pike Esox lucius in Lithuania
  113. In vitro sensitivity of Blastocystis hominis to garlic, ginger, white cumin, and black pepper used in diet
  114. Cuticular Architecture of Hassalstrongylus epsilon (Nematoda: Trichostrongyloidea)
  115. Recruitment rate of gymnophallid metacercariae in the New Zealand cockle Austrovenus stutchburyi : an experimental test of the hitch-hiking hypothesis
  116. Comparative proteomic analysis of trophozoites versus cysts of Giardia lamblia
  117. Increased resistance to anthelmintics of Haemonchus contortus eggs associated with changes in membrane fluidity of eggshells during embryonation
Search Result: