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Title of Journal: Parasitol Res

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Abbravation: Parasitology Research

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Springer Berlin Heidelberg

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DOI

10.1007/bf00817657

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1432-1955

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The siRNAmediated silencing of Emphasis Type="It

Authors: Zhong Quan Wang Shuai Bing Zhang Peng Jiang Ruo Dan Liu Shao Rong Long Xi Zhang Hui Jun Ren Jing Cui
Publish Date: 2015/08/02
Volume: 114, Issue: 9, Pages: 3551-3557
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Abstract

Previous studies showed that Trichinella spiralis Nudix hydrolase TsNd bound to intestinal epithelial cells IECs and vaccination of mice with rTsNd or TsNd DNA produced a partial protective immunity against T spiralis infection In this study three TsNd specific small interfering RNA siRNA were designed to silence the expression of TsNd in T spiralis larvae SiRNAs were delivered to the larvae by electroporation Silencing effect of TsNd transcription and expression was determined by realtime PCR and Western blotting respectively The infectivity of the larvae treated with siRNA was investigated by the in vitro larval invasion of IECs and experimental infection in mice The results showed that siRNAs were efficiently delivered into T spiralis larvae through electroporation Realtime PCR and Western blotting showed that transcription and expression level of TsNd gene was inhibited 733 and 767  respectively after being electroporated with 2 μM of siRNA275 for 1 day Silencing TsNd expression inhibited significantly the larval invasion of IECs P  001 and was in a dosedependent manner r = −097941 The mice with infected larvae treated with TsNd siRNA displayed a 636  reduction in intestinal adult worms and 688  reduction in muscle larval burden compared with mice infected with control siRNAtreated larvae Our results showed that silencing TsNd expression in T spiralis significantly reduced the larval infectivity and survival in host

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