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Title of Journal: Parasitol Res

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Abbravation: Parasitology Research

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Springer-Verlag

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DOI

10.1007/s10682-016-9868-x

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1432-1955

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Larvicidal activity of silver nanoparticles synthe

Authors: Chandrashekhar D Patil Hemant P Borase Satish V Patil Rahul B Salunkhe Bipinchandra K Salunke
Publish Date: 2012/02/28
Volume: 111, Issue: 2, Pages: 555-562
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Abstract

In present study the bioactivity of latexproducing plant Pergularia daemia as well as synthesized silver nanoparticles AgNPs against the larval instars of Aedes aegypti and Anopheles stephensi mosquito larvae was determined The range of concentrations of plant latex 1000 500 250 125 6225 and 3125 ppm and AgNPs 10 5 25 125 0625 and 03125 ppm were prepared The LC50 and LC90 values for first second third and fourth instars of synthesized AgNPstreated first second third and fourth instars of A aegypti LC50 = 439 512 566 618 LC90 = 990 1113 1240 1295 ppm and A stephensi LC50 = 441 535 591 647 LC90 = 1010 1204 1305 1408 ppm were found many fold lower than crude latextreated A aegypti LC50 = 5513 5881 7566 9431 LC90 = 11300 11825 15695 17571 ppm and A stephensi LC50 = 8147 9209 9607 10131 LC90 = 15951 17597 18067 19042 ppm The AgNPs did not exhibit any noticeable effects on Poecillia reticulata after either 24 or 48 h of exposure at their LC50 and LC90 values against fourthinstar larvae of A aegypti and A stephensi The UVvisible analysis shows absorbance for AgNPs at 520 nm TEM reveals spherical shape of synthesized AgNPs Particle size analysis revealed that the size of particles ranges from 44 to 255 nm with average size of 12350 nm AgNPs were clearly negatively charged zeta potential −274 mV This is the first report on mosquito larvicidal activity P daemiasynthesized AgNPs

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