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Title of Journal: Parasitol Res

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Abbravation: Parasitology Research

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Springer-Verlag

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1432-1955

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Emphasis Type="Italic"Trichobilharzia/Emphasis

Authors: Damien Jouet Hubert Ferté Jérôme Depaquit Jitka Rudolfová Pierre Latour Damien Zanella Matthieu L Kaltenbach Nicole Léger
Publish Date: 2008/03/05
Volume: 103, Issue: 1, Pages: 51-
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Abstract

Annecy Lake is a wellknown focus of human cercarial dermatitis in France Identification of the parasites however was not performed in the past Previous studies suspected two species Trichobilharzia franki and Trichobilharzia regenti based on the presence of parasites in mallards and/or morphological identification of snails emitting ocellate furcocercariae Following a standardized molecular approach we studied snails and furcocercariae and compared their haplotypes with those deposited in GenBank The selected markers were the second internal transcribed spacer ITS2 for the snails and ITS2 and D2 domain of the ribosomal DNA for the parasites Our results confirm the presence of T franki and T regenti and two probable new species that could be potential agents of cercarial dermatitis All the snails emitting the ocellate furcocercariae belong to the same species identified as Radix peregra =Radix ovata = Radix balthica Parasite–host relationships between species of the genus Trichobilharzia and snails of the genus Radix do not seem to be as specific as supposed previouslyThe authors thank Monique Boutry Chantal Grimplet Emmanuel Liénard and the technical staff of Syndicat Mixte du Lac d’Annecy SILA for their technical assistance and Bernard Pesson for proofreading this manuscript Financial support for this study was provided by SILA and Office National de la Chasse et de la Faune Sauvage by the Czech Ministry of Education Grants No MSM0021620828 and No MSM LC06009 and by the Czech Science Foundation Grant No 206/07/P092

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