Journal Title
Title of Journal: Parasitol Res
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Abbravation: Parasitology Research
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Publisher
Springer Berlin Heidelberg
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Authors: Jun Sun SuWen Wang Chen Li Wei Hu YiJiu Ren JinQiang Wang
Publish Date: 2013/12/03
Volume: 113, Issue: 3, Pages: 881-892
Abstract
Pairing of Schistosoma japonicum initiates female development leads to female sexual maturation and maintains this mature state To understand the mechanism involved in these processes we studied parasites isolated from single and doublesex cercariaeinfected mice using deepsequencing analysis Solexa to uncover pairregulated transcriptional profiles In this study we report the results of highthroughput tagsequencing Tagseq analysis of the transcriptome of female worms 18 and 23 days postsingle and doublesex infections We sequenced over 3 million tags obtained a total of 14034 27251 22755 and 22555 distinct tags corresponding to 5773 9794 8885 and 8870 tagmapped genes for 23dayold female schistosomula from doublesex infections 23DSI 23dayold female schistosomula from singlesex infections 23SSI 18dayold female schistosomula from doublesex infections 18DSI and 18dayold female schistosomula from singlesex infections 18SSI respectively Analyses of differentially expressed genes revealed similarities in the gene expression profiles between 18SSI and 18DSI as well as rational differential gene expression between 18SSI and 23SSI However fewer upregulated genes were found in 23DSI compared with 18DSI Of the 3446 differentially expressed genes between 23DSI and 23SSI 2913 genes were upregulated in 23SSI whereas only 533 genes were upregulated in 23DSI In these upregulated genes in 23DSI phosphoglycerate mutase superoxide dismutase egg antigen ribosomal proteins ferritin1 heavy chain and eukaryotic translation initiation factor 2 were detected Detection of these genes suggests that gene expression in 23DSI is specialized for functions such as promotion and maintenance of female sexual maturation and egg production Quantitative realtime RTPCR analysis confirmed the Solexa results thereby supporting the reliability of the system Our results offer new insights into the biological significance of pairing which directs the expression of genes specific for sexual maturation and egg production
Keywords:
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