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Title of Journal: Parasitol Res

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Abbravation: Parasitology Research

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Springer-Verlag

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DOI

10.1007/bf03060571

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1432-1955

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Excretory/secretory products of sheep abomasal nem

Authors: Sabine Przemeck Alexandra Huber Simon Brown Kevin C Pedley Heather V Simpson
Publish Date: 2005/01/27
Volume: 95, Issue: 3, Pages: 213-217
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Abstract

Excretory/secretory ES products of Ostertagia Teladorsagia circumcincta and Haemonchus contortus have been implicated in the inhibition of gastric acid secretion and vacuolation and the loss of parietal cells associated with abomasal parasitism Vacuolation of epithelial HeLa cells caused by adult O circumcincta or L3 O circumcincta or H contortus ES products have been examined by differential interference contrast microscopy and by the neutral red uptake assay ES products caused visible vacuolation of HeLa cells and this effect was enhanced by 8 mM NH4Cl Some parasite ES products caused a marked detachment of cells from the coverslip At lower concentrations of ES products neutral red uptake was usually increased above the control but at higher concentrations of ES products uptake was often decreased probably because of cell detachment Although generally consistent with direct observations of HeLa cell vacuolation by parasite chemicals neutral red uptake was not a satisfactory quantitative assay

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