Authors: S Alasaad L Rossi R C Soriguer L Rambozzi D Soglia J M Pérez X Q Zhu
Publish Date: 2009/01/22
Volume: 104, Issue: 4, Pages: 723-732
Abstract
Sarcoptes mite from collection to DNA extraction forms the cornerstone for studies on Sarcoptes scabiei Whilst the new science era took a shy leap into the different facets of mite studies the cornerstone was almost entirely neglected Mite collection cleaning storage and DNA extraction were basically humble attempts to extrapolate adapt modify or ‘pirate’ those existing methods to the peculiarities of Sarcoptes research These aspects usually constituted few lines bashfully mentioned in the materials and methods section of some papers which arose in unique problems concerning costeffectiveness time profitability safety and even worse the credibility of the results creating contradictory conclusions in some cases This ‘noisy’ situation encouraged us to collect classify and review for the first time to our knowledge some aspects relating to studies on Sarcoptes mite from collection to DNA extraction which will be useful for further studies on Sarcoptes and have implications for the effective control of the diseases Sarcoptes mite causes Further studies are needed especially to compare the profitability safety sensibility and specificity of the different methods of this neglected realm of the ubiquitous ectoparasiteThe authors thank Aleppo UniversitySyria Università di TorinoItaly Universidad de JaénSpain Estación Biológica de Doñana CSICSpain RNM118 investigation group Junta de AndalucíaSpain and South China Agricultural University—China for financial support and fellowships dedicated to parasitology research XQZ is supported by grants from the Program for Changjiang Scholars and Innovative Research Team in University Grant No IRT0723 and the Natural Science Foundation of Guangdong Province Team Project Grant no 5200638
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