Authors: E De Menis G Tulipano S Villa D Billeci C Bonfanti P Pollara P Pauletto Andrea Giustina
Publish Date: 2014/03/18
Volume: 26, Issue: 4, Pages: 359-363
Abstract
Somatostatin receptors are highly expressed in almost all meningiomas but in this setting their functional role is not clear A 59yrold woman had been treated with octreotide after an unsuccessful operation for a GHsecreting pituitary adenoma After 8 yr of treatment a nuclear magnetic resonance NMR scan disclosed a 3 cm meningioma of the tentorium Mean GH was 22 ng/ml and IGFI 325 ng/ml Meningioma was resected and tissue was digested to obtain tumor cell suspension Aim of the study was to measure epidermal growth factor EGFinduced proliferation of cultured meningioma cells in the presence of either somatostatin or octreotide Cells were grown to semiconfluency in Dolbecco’s modified eagle medium DMEM supplemented with 10 fetal calf serum FCS After 48 h in DMEM without serum the medium was replaced by fresh medium plus recombinant EGF 10 ng/ml and somatostatin or octreotide were added in the final concentrations of 110 and 100 nM 20 h later 1 μcgCi of 3Hthymidine was added to each well After 4 h incorporated radioactivity was measured While octreotide did not influence significantly cell growth at the three dose tested somatostatin increased thymidine incorporation dosedependently peak 100nM 150±27 vs medium plus EGF p005 Octreotide effectively suppressed GH secretion in our acromegalic patient but is unlikely that its longterm use could have stimulated the growth of meningioma since it did not significantly influence the in vitro proliferation of the meningioma cells These results suggest that somatostatinmediated proliferative effect on meningioma cells is not mediated by the subtype 2 of the somatostatin receptor
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