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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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DOI

10.1002/chin.200129111

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1432-0614

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Synthesis of flavor and fragrance esters using Em

Authors: Araceli Larios Hugo S García Rosa María Oliart Gerardo ValerioAlfaro
Publish Date: 2004/07/10
Volume: 65, Issue: 4, Pages: 373-376
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Abstract

Candida antarctica lipase fraction B CALB showed substrate specificity in the synthesis of esters in hexane involving reactions of shortchain acids having linear acetic and butyric acids and branched chain isovaleric acid structures an unsaturated tiglic acid fatty acid and phenylacetic acid with nbutanol and geraniol The variation in the conversion to the esters was ca 10 Similar results were observed in a study of the alcohol specificity of the enzyme for esterification of acetic and butyric acids with four alcohols nbutyl isopentyl 2phenylethyl and geraniol Enantioselectivity of CALB in hexane with a range of chiral αsubstituted or βsubstituted carboxylic acids and nbutyl alcohol was analyzed The results show that CALB can be employed as a robust biocatalyst in esterification reactions due to the high conversions obtained in the synthesis of shortchain flavor esters in an organic solvent although this enzyme exhibited modest enantioselectivity with chiral shortchain carboxylic acids

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