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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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DOI

10.1002/pmic.201470031

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1432-0614

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Production of a cytotoxic cationic antibacterial p

Authors: Jian Feng Li Jie Zhang Ren Song Jia Xin Zhang Yang Shen Shuang Quan Zhang
Publish Date: 2009/07/07
Volume: 84, Issue: 2, Pages: 383-388
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Abstract

Antibacterial peptide CM4 ABPCM4 is a small cationic peptide with broadspectrum activities against bacteria fungi and tumor cells which may possibly be used as an antimicrobial agent We report here the application of small ubiquitinrelated modifier SUMO fusion technology to the expression and purification of cationic antibacterial peptide ABPCM4 The fusion protein expressed in a soluble form was purified to a purity of 90 by NiIDA chromatography and 112 mg protein of interest was obtained per liter of fermentation culture After the SUMO–CM4 fusion protein was cleaved by the SUMO protease at 30 °C for 1 h the cleaved sample was reapplied to a NiIDA Finally about 24 mg recombinant CM4 was obtained from 1 l fermentation culture with no less than 96 purity and the recombinant CM4 had similar antimicrobial properties to the synthetic CM4 Thus the SUMOmediated peptide expression and purification system potentially could be employed for the production of recombinant cytotoxic peptides

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