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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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DOI

10.1007/s00109-002-0400-0

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1432-0614

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Characterization of a novel xylanase from Emphasi

Authors: Saurabh Sudha Dhiman Dayanand Kalyani Sujit Sadashiv Jagtap JungRim Haw Yun Chan Kang JungKul Lee
Publish Date: 2012/09/07
Volume: 97, Issue: 3, Pages: 1081-1091
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Abstract

Enhanced catalytic activities of different lignocellulases were obtained from Armillaria gemina under statistically optimized parameters using a jar fermenter This strain showed maximum xylanase endoglucanase cellobiohydrolase and βglucosidase activities of 1270 146 34 and 15 U mL−1 respectively Purified A gemina xylanase AgXyl has the highest catalytic efficiency k cat/K m = 1440 mg mL−1 s−1 ever reported for any fungal xylanase highlighting the significance of the current study We covalently immobilized the crude xylanase preparation onto functionalized silicon oxide nanoparticles achieving 117  immobilization efficiency Further immobilization caused a shift in the optimal pH and temperature along with a fourfold improvement in the halflife of crude AgXyl Immobilized AgXyl gave 378  higher production of xylooligosaccharides compared to free enzyme After 17 cycles the immobilized enzyme retained 92  of the original activity demonstrating its potential for the synthesis of xylooligosaccharides in industrial applicationsThis research was supported by the Converging Research Center Program through the National Research Foundation of Korea funded by the Ministry of Education Science and Technology grant 2011–50210 This study was also supported by Brain Pool 2011 of Konkuk University Republic of Korea

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