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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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10.1002/cber.187600901240

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1432-0614

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Enhanced vanillin production from ferulic acid usi

Authors: Dongliang Hua Cuiqing Ma Lifu Song Shan Lin Zhaobin Zhang Zixin Deng Ping Xu
Publish Date: 2006/11/24
Volume: 74, Issue: 4, Pages: 783-790
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Abstract

High vanillin productivity was achieved in the batch biotransformation of ferulic acid by Streptomyces sp strain V1 Due to the toxicity of vanillin and the product inhibition fedbatch biotransformation with high concentration of ferulic acid was unsuccessful To solve this problem and improve the vanillin yield a biotransformation strategy using adsorbent resin was investigated Several macroporous adsorbent resins were chosen to adsorb vanillin in situ during the bioconversion Resin DM11 was found to be the best which adsorbed the most vanillin and the least ferulic acid When 8 resin DM11 wet w/v was added to the biotransformation system 45 g l−1 ferulic acid could be added continually and 192 g l−1 vanillin was obtained within 55 h which was the highest vanillin yield by bioconversion until now This yield was remarkable for exceeding the crystallization concentration of vanillin and therefore had farreaching consequence in its downstream processingThe authors gratefully acknowledged the financial support of this work by Shanghai Apple Flavor Fragrance China The work was supported in part by the financial support of the State Major Basic Research Development Program China grant 2007CB707800

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