Authors: Kim Heylen Sven Hoefman Bram Vekeman Jindrich Peiren Paul De Vos
Publish Date: 2012/03/14
Volume: 94, Issue: 3, Pages: 565-574
Abstract
Environmental research delivers valuable bacterial resources for biotechnology We believe that systematic longterm preservation of bacteria will promote future biotechnological innovations by safeguarding the accessibility of bacteria already recognized to have interesting features and providing a “pool” of bacterial resources for novel applied research To this end we want to advocate the incorporation of preservation tests in environmental or applied microbiological research This paper introduces nonspecialists to different preservation methods for bacteria Several parameters that influence longterm storage of bacterial resources are explained and practical tips and guidelines are formulated Also the vital role of public culture collections is highlighted and the stateoftheart of preservation of nonpure cultures is describedThis work was funded by the Geconcerteerde Onderzoeksactie GOA of Ghent University BOF09/GOA/005 The authors are grateful to Dr Eva Spieck and Dr Andreas PömmereningRoeser Hamburg University Germany for providing nitrite and ammoniaoxidizing strains which were used in unpublished preservation research
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