Authors: Fang Zhang JingHua Yang Kun Dai Yun Chen QiuRong Li FaMing Gao Raymond J Zeng
Publish Date: 2015/11/13
Volume: 100, Issue: 3, Pages: 1511-1521
Abstract
The microbial community compositions of a chemostat enriched in a thermophilic 55 °C mixed culture fermentation MCF for hydrogen production under different operational conditions were revealed in this work by integrating denaturing gradient gel electrophoresis DGGE Illumina Miseq highthroughput sequencing and 16S rRNA clone library sequencing The results showed that the community structure of the enriched cultures was relatively simple Clones close to the genera of Thermoanaerobacter and/or Bacillus mainly dominated the bacteria And homoacetogens and archaea were washed out and not detected even by Illumina Miseq highthroughput sequencing which supported the benefit for hydrogen production On the other hand the results revealed that the metabolic shift was clearly associated with the change of dominated bacterial groups The effects of hydrogen partial pressure PH2 and pH from 40 to 55 on the microbial compositions were not notable and Thermoanaerobacter was dominant thus the metabolites were also not changed While Bacillus Thermoanaerobacter and Propionispora hippei dominated the bacteria communities at neutral pH or Bacillus and Thermoanaerobacter dominated at high influent glucose concentrations consequently the main metabolites shifted to acetate ethanol propionate or lactate Thereby the effect of microbial composition on the metabolite distribution and shift shall be considered when modeling thermophilic MCF in the futureThe authors would like to acknowledge the financial support from the National Natural Science Foundation of China 51408530 50978244 the China Postdoctoral Science Foundation 2015 T80233 the Natural Science Foundation of Hebei Province E2015203306 the Postdoctoral Science Foundation of Hebei Province B2014003008 the National HiTechnology Development 863 Program of China 2011AA060901 the HundredTalent Program of Chinese Academy of Sciences the Program for Changjiang Scholars and Innovative Research Team in University and the Fundamental Research Funds for the Central Universities wk2060190040
Keywords: