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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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1432-0614

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Heterologous expression of metabotropic glutamate

Authors: K Sugiyama T P Niki K Inokuchi Y Teranishi M Ueda A Tanaka
Publish Date: 2004/01/22
Volume: 64, Issue: 4, Pages: 531-536
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Abstract

The upstream region of the isocitrate lyase gene UPRICL from the nalkaneutilizing yeast Candida tropicalis serves as a useful promoter of gene expression in the yeast Saccharomyces cerevisiae The production of rat metabotropic glutamate receptor 1α mGluR1α which belongs to the Gproteincoupled receptor GPCR family was tested under the control of UPRICL Expression of mGluR1α was found in recombinant clones and enhanced by replacing the signal sequence of mGluR1α with the corresponding region of the αfactor receptor Ste2 which is a GPCR found in S cerevisiae Moreover the membrane fraction from a recombinant clone associated with Vesl1S/Homer1a protein binds the mGluR1α in rat cerebellum These results suggest that the UPRICLcontrolled gene expression system is useful for heterologous GPCRs in S cerevisiae

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