Authors: Jingjing Liu Guixin Yan Changlong Shu Can Zhao Chunqin Liu Fuping Song Lin Zhou Junlan Ma Jie Zhang Dafang Huang
Publish Date: 2010/02/18
Volume: 87, Issue: 1, Pages: 243-249
Abstract
A newly engineered strain denominated BIOT185 was constructed through integrating the cry8Ca2 gene into the endogenous plasmid of BT185 contains cry8Ea1 by homologous recombination The thermosensitive plasmid vector was eliminated by the rising temperature of recombinant cultures No antibiotic gene or other unnecessary genes were introduced to the new strain Sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blot analysis demonstrated that the cry8Ca2 gene was expressed normally and produced a 130kDa protein in the BIOT185 strain Bioassay results showed that the new strain had high toxicity to the pests Anomala corpulenta and Holotrichia parallela which often damage the same fields
Keywords: