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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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DOI

10.1007/s00220-012-1436-9

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1432-0614

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Selection of optimal reference genes for expressio

Authors: YongHong Zhou YongJun Zhang ZhiBing Luo YanHua Fan GuiRong Tang LiJi Liu Yan Pei
Publish Date: 2011/09/16
Volume: 93, Issue: 2, Pages: 679-685
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Abstract

The selection of suitable reference genes is crucial for accurate quantification of gene expression To identify suitable reference genes in Beauveria bassiana the expression of 14 candidates 18S 28S βTub GAPD γAct TEF HGPT His3 His2A TBP CypA CypB PP1 and CrzA was measured by quantitative polymerase chain reaction at different development stages and under various nutritional and stress conditions Expression stability as evaluated by the geNorm and NormFinder programs revealed that His2A/γAct/CrzA was the most stably expressed set of genes throughout development while 28S/PP1/CypA and His2A/γAct/CypA were the most stably expressed gene sets under a variety of nutritional and stress conditions respectively Overall the most stably expressed genes under all conditions examined were PP1 γAct and CypAThis work was supported by grants from the Ministry of Science and Technology of China 2009CB118904 The authors would like to thank GuangMao Shen for geNorm and NormFinder assistance and JinCheng Ma XiuPing Zou and XiaoDong Jiang for critical reading of the manuscript

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