Authors: Han Meng YongFeng Wang HoWang Chan RuoNan Wu JiDong Gu
Publish Date: 2016/05/13
Volume: 100, Issue: 17, Pages: 7727-7739
Abstract
Anaerobic ammonium oxidation anammox and nitritedependent anaerobic methane oxidation ndamo are two new processes of recent discoveries linking the microbial nitrogen and carbon cycles In this study 16S ribosomal RNA rRNA gene of anammox bacteria and pmoA gene of ndamo bacteria were used to investigate their distribution and diversity in natural acidic and revegetated forest soils The 16S rRNA gene sequences retrieved featured at least three species in two genera known anammox bacteria namely Candidatus Brocadia anammoxidans Candidatus Brocadia fulgida and Candidatus Kuenenia stuttgartiensis while the pmoA gene amplified was affiliated with two species of known ndamo bacteria Candidatus Methylomirabilis oxyfera and a newly established Candidatus Methylomirabilis sp According to the results the diversity of anammox bacteria in natural forests was lower than in revegetated forests but no significant difference was observed in ndamo community between them Quantitative realtime PCR showed that both anammox and ndamo bacteria were more abundant in the lower layer 10–20 cm than the surface layer 0–5 cm The abundance of anammox bacteria varied from 221 × 105 to 390 × 106 gene copies per gram dry soil and ndamo bacteria quantities were between 169 × 105 and 507 × 106 gene copies per gram dry soil in the two different layers Both anammox and ndamo bacteria are reported for the first time to cooccur in acidic forest soil in this study providing a more comprehensive information on more defined microbial processes contributing to C and N cycles in the ecosystemsThis project was supported by the National Natural Science Foundation of China grant No 31470562 to YFW Hong Kong PhD Fellowship HM and RGC GRF Grant No 701913 JDG Additional financial support for this research project was from the laboratory fund
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