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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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DOI

10.1007/978-1-4939-0348-1_16

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1432-0614

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Protein engineering opportunities and challenges

Authors: Matti Leisola Ossi Turunen
Publish Date: 2007/04/03
Volume: 75, Issue: 6, Pages: 1225-1232
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Abstract

The extraordinary properties of natural proteins demonstrate that lifelike protein engineering is both achievable and valuable Rapid progress and impressive results have been made towards this goal using rational design and random techniques or a combination of both However we still do not have a general theory on how to specify a structure that is suited to a target function nor can we specify a sequence that folds to a target structure There is also overreliance on the Darwinian blind search to obtain practical results In the long run random methods cannot replace insight in constructing lifelike proteins For the near future however in enzyme development we need to rely on a combination of both

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