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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer Berlin Heidelberg

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DOI

10.1002/jhet.5570190655

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1432-0614

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An exceptionally coldadapted alphaamylase from a

Authors: Jan Kjølhede Vester Mikkel Andreas Glaring Peter Stougaard
Publish Date: 2014/07/20
Volume: 99, Issue: 2, Pages: 717-727
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Abstract

A coldactive αamylase AmyI3C6 identified by a functional metagenomics approach was expressed in Escherichia coli and purified to homogeneity Sequence analysis showed that the AmyI3C6 amylase was similar to αamylases from the class Clostridia and revealed classical characteristics of coldadapted enzymes as did comparison of the kinetic parameters K m and k cat to a mesophilic αamylase AmyI3C6 was shown to be heatlabile Temperature optimum was at 10–15 °C and more than 70  of the relative activity was retained at 1 °C The pH optimum of AmyI3C6 was at pH 8–9 and the enzyme displayed activity in two commercial detergents tested suggesting that the AmyI3C6 αamylase may be useful as a detergent enzyme in environmentally friendly lowtemperature laundry processesThe Government of Greenland is thanked for the permission to sample ikaite material Novozymes A/S Denmark is acknowledged for the Stainzyme® used This work was supported by a grant to JKV from University of Copenhagen and to PS from the Danish Environmental Protection Agency

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