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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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DOI

10.1002/cphc.201601056

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1432-0614

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Glutathione accumulation in ethanolstat fedbatch

Authors: Ildar Nisamedtinov Kaspar Kevvai Kerti Orumets Jari J Rautio Toomas Paalme
Publish Date: 2010/03/10
Volume: 87, Issue: 1, Pages: 175-183
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Abstract

Shotwise supplementation of cysteine to a yeast culture is a common means of promoting glutathione GSH production In the present work we study the accumulation kinetics of cysteine γglutamylcysteine and GSH and the expression of genes involved in GSH and sulfur metabolism in ethanolstat fedbatch cultures as a result of switching to a medium enriched with cysteine and glycine Supplementation in this fashion resulted in a rapid but shortterm increase in the rate of GSH synthesis while the expression of GSH1 decreased Expression of GSH1 and GSH synthesis rate were observed to revert close to the base level after a few hours These results indicate that under such conditions the control of GSH synthesis at higher concentrations occurred at the enzymatic rather than the transcriptional level The incorporation of cysteine into GSH was limited to ∼40 of the theoretical yield due to its requirement as a source of sulfur for protein synthesis under conditions whereby the sulfate assimilation pathway is downregulated This was supported by the expression profiles of genes involved in cysteine and homocysteine interconversionThe financial support for this research was provided by the Enterprise Estonia project EU22704 Estonian Ministry of Education and Research grant SF0140090s08 and by Estonian Science Foundation grant G7323 We would like to thank Mr David Schryer and Dr Chris Powell for critical revision of the manuscript

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