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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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DOI

10.1007/s11146-009-9174-2

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1432-0614

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Biosynthesis of ethylene glycol in Emphasis Type=

Authors: Huaiwei Liu Kristine Rose M Ramos Kris Niño G Valdehuesa Grace M Nisola WonKeun Lee WookJin Chung
Publish Date: 2012/12/12
Volume: 97, Issue: 8, Pages: 3409-3417
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Abstract

Ethylene glycol EG is an important platform chemical with steadily expanding global demand Its commercial production is currently limited to fossil resources no biosynthesis route has been delineated Herein a biosynthesis route for EG production from dxylose is reported This route consists of four steps dxylose → dxylonate → 2dehydro3deoxydpentonate → glycoaldehyde → EG Respective enzymes dxylose dehydrogenase dxylonate dehydratase 2dehydro3deoxydpentonate aldolase and glycoaldehyde reductase were assembled The route was implemented in a metabolically engineered Escherichia coli in which the dxylose → dxylulose reaction was prevented by disrupting the dxylose isomerase gene The most efficient construct produced 117 g L−1 of EG from 400 g L−1 of dxylose Glycolate is a carboncompeting byproduct during EG production in E coli blockage of glycoaldehyde → glycolate reaction was also performed by disrupting the gene encoding aldehyde dehydrogenase but from this approach EG productivity was not improved but rather led to dxylonate accumulation To channel more carbon flux towards EG than the glycolate pathway further systematic metabolic engineering and fermentation optimization studies are still required to improve EG productivityThis work was supported by Priority Research Centers Program through the National Research Foundation of Korea funded by the Ministry of Education Science and Technology 2012–0006693 The authors would like to thank Professor Chankyu Park from Korea Advanced Institute of Science and Technology KAIST for generously providing the pET15ayqhD plasmid and Professor Jaejoon Han from Sungkyunkwan University for kindly providing a reagent for the enzyme activity assays

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