Authors: Pei Huang Feng Xie Biao Ren Qian Wang Jian Wang Qi Wang Wael M AbdelMageed Miaomiao Liu Jianying Han Ayokunmi Oyeleye Jinzhao Shen Fuhang Song Huanqin Dai Xueting Liu Lixin Zhang
Publish Date: 2016/03/15
Volume: 100, Issue: 17, Pages: 7437-7447
Abstract
Microbes belonging to the genus Verrucosispora possess significant chemical diversity and biological properties They have attracted the interests of many researchers and are becoming promising resources in the marine natural product research field A bioassayguided isolation from the crude extract of Verrucosispora sp strain MS100047 isolated from sediments collected from the South China Sea has led to the identification of a new salicylic derivative glycerol 1hydroxy25dimethyl benzoate 1 along with three known compounds brevianamide F 2 abyssomicin B 3 and proximicin B 4 Compound 1 showed selective activity against methicillinresistant Staphylococcus aureus MRSA with a minimum inhibitory concentration MIC value of 125 μg/mL Brevianamide F 2 which was isolated from actinomycete for the first time showed a good antiBCG activity with a MIC value of 125 μg/mL that has not been reported previously in literatures Proximicin B 4 showed significant antiMRSA MIC = 3125 μg/mL antiBCG MIC = 625 μg/mL and antituberculosis TB MIC = 25 μg/mL activities This is the first report on the antitubercular activities of proximicins In addition Verrucosispora sp strain MS100047 was found to harbor 18 putative secondary metabolite gene clusters based on genomic sequence analysis These include the biosynthetic loci encoding polyketide synthase PKS and nonribosomal peptide synthetase NRPS consistent with abyssomicins and proximicins respectively The biosynthetic pathways of these isolated compounds have been proposed These results indicate that MS100047 possesses a great potential as a source of active secondary metabolitesThis work was supported by the National Program on Key Basic Research Project 973 program 2013CB734000 by grants from the China Ocean Mineral Resources RD Association DY12515T07 the National Natural Science Foundation of China 81102369 31430002 31320103911 the Ministry of Science and Technology of China 2013ZX10005004005 and 2011ZX0910201111 and the European Union’s Seventh Framework Programme FP7/2007–2013 under grant agreement no 312184 Wael M AbdelMageed is an awardee of the fellowship for young International Scientists of Chinese Academy of Sciences CAS 31050110430
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