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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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DOI

10.1007/s12459-012-0004-x

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1432-0614

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Separation of ionic liquid MmimDMP and glucose

Authors: Dexin Feng Liangzhi Li Fang Yang Weiqiang Tan Guoming Zhao Huibin Zou Mo Xian Yingwei Zhang
Publish Date: 2011/05/27
Volume: 91, Issue: 2, Pages: 399-405
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Abstract

Pretreatment of cellulose with ionic liquids ILs can improve the efficiency of the hydrolysis by increasing the surface area of the substrates accessible to solvents and cellulases However the IL methods are facing challenges to separate the hydrolyzed sugar products as well as the renewable ILs from the complex hydrolysis mixtures In this study an alumina column chromatography ACC method was developed for the separation of hydrophilic IL NmethylNmethylimidazolium dimethyl phosphate MmimDMP and glucose which was the main ingredient of the monosaccharide hydrolyzate The processing parameters involved in ACC separation were investigated in detail Our results showed that the recovery yields of MmimDMP and glucose can reach up to 9338 and 9014 respectively under the optimized parameters the sampling ratio of 120 between the applied sample volume and the bed volume of the column a gradient elution using methanol 100 150 ml and then water 170 ml as eluents with 1 ml/min flow rate The recovered MmimDMP showed qualified property and was effective in a new hydrolysis reaction In addition scaleup ACC separations were successfully done with satisfied separation performance The results indicated that the ACC is one of the available methods for the separation of ILs and monosaccharides from the hydrolysis mixtures

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