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Title of Journal: Appl Microbiol Biotechnol

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Abbravation: Applied Microbiology and Biotechnology

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Springer-Verlag

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DOI

10.1006/icar.2002.6926

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1432-0614

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Metabolic flux distributions genetic information

Authors: Lars M Blank Lars Kuepfer
Publish Date: 2010/03/16
Volume: 86, Issue: 5, Pages: 1243-1255
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Abstract

Flux distributions in intracellular metabolic networks are of immense interest to fundamental and applied research since they are quantitative descriptors of the phenotype and the operational mode of metabolism in the face of external growth conditions In particular fluxes are of relevance because they do not belong to the cellular inventory eg transcriptome proteome metabolome but are rather quantitative moieties which link the phenotype of a cell to the specific metabolic mode of operation A frequent application of measuring and redirecting intracellular fluxes is strain engineering which ultimately aims at shifting metabolic activity toward a desired product to achieve a high yield and/or rate In this article we first review the assessment of intracellular flux distributions by either qualitative or rather quantitative computational methods and also discuss methods for experimental measurements The tools at hand will then be exemplified on strain engineering projects from the literature Finally the achievements are discussed in the context of future developments in Metabolic Engineering and Synthetic BiologyWe thank Birgitta Ebert for the valuable comments on the manuscript and Jana Rühl for critically reading the genetic information section LMB acknowledges the ongoing support of Andreas Schmid We apologize to all researchers who also contributed significantly to the advances of metabolic flux analysis and prediction but were not mentioned due to space restrictions

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