Authors: Jingbo Tang Xingyan Liu Jian Peng Yong Tang Yang Zhang
Publish Date: 2015/02/17
Volume: 99, Issue: 6, Pages: 2763-2772
Abstract
A marinederived actinobacteria Streptomyces sp M10 was identified as a prolific antifungal compounds producer and shared a 9902 16S ribosomal RNA rRNA sequence similarity with that of Streptomyces marokkonensis Ap1T which can produce polyene macrolides To further evaluate its biosynthetic potential the 7207169 bp Streptomyces sp M10 linear chromosome was sequenced and mined for identifiable secondary metaboliteassociated gene clusters A total of 20 secondary metaboliteassociated gene clusters were deduced including three polyketide synthases PKSs four nonribosomal peptide synthetases NRPSs four hybrid NRPSPKSs three NRPSindependent siderophores and two lantibiotic and four terpene biosynthetic gene clusters One of the type I PKS gene cluster pks1 shared a 85 nucleotide similarity with candicidin/FR008 gene cluster indicating the capacity of this organism to produce polyene macrolides This assumption was verified by a scaleup culturing of Streptomyces sp M10 on A1 agar plates which lead to the isolation of two polyene families PF1 and PF2 with characteristic UV adsorption at 269 278 and 290 nm PF1 and 363 386 and 408 nm PF2 respectively Compound 904 was further purified from PF1 and its chemical structure was partially elucidated to be a typical polyene macrolide by NMR and UV spectrum This study affirmatively identified Streptomyces sp M10 as a source of polyene metabolites and highlighted genome mining of interested organism as a powerful tool for natural product discoveryThe EditorinChief and the Associate Editors have decided to retract the this article Upon investigation carried out according to the Committee on Publication Ethics guidelines it has been found that the authors submitted an unpublished paper of which they are not the original authors The authors admitted their misconduct and agreed to retract this article
Keywords: