Authors: Mamun M OrRashid Ousama AlZahal Brian W McBride
Publish Date: 2008/09/17
Volume: 81, Issue: 3, Pages: 533-541
Abstract
The present study was designed to investigate the capability of mixed rumen protozoa to synthesize conjugated linoleic acid CLA from linoleic LA and vaccenic acids VA Rumen contents were collected from fistulated cows The protozoal fraction was separated and washed several times with MB9 buffer and then resuspended in autoclaved rumen fluid The suspensions were anaerobically incubated up to 18 h at 385 °C with substrates in the presence PAB or the absence of antibacterialagents PNoAB Neither PAB nor PNoAB suspensions were capable of producing CLA from VA 11t181 Linoleic acid was catabolized by PNoAB to a greater extent than PAB Different isomers of CLA were synthesized by PAB from LA The 9c11tCLA was predominant Thirty seven percent of the maximum accumulated 9c11tCLA was found in the PAB suspension as early as 01 h into the incubation period Accumulation of 10t12cCLA in PAB suspension was approximately 100 times lower than that of 9c11tCLA There were no significant productions of VA 10t181 and 180 in PAB compared with the control indicating that rumen protozoa have no ability to biohydrogenate CLA isomers On the other hand the concentrations of 10t181 VA and 180 in PNoAB were greater P 005 compared with those in PAB indicating the role of symbiotic bacteria associated with PNoAB in biohydrogenating CLA isomers We concluded that mixed rumen protozoa are capable of synthesizing CLA from LA through isomerization reactions However they are incapable of metabolizing CLA further They are also incapable of vaccenic acid biohydrogenation and/or desaturationThe authors would like to thank the staff at the Elora Dairy Research Centre University of Guelph ON Canada for their technical assistance We would also like to acknowledge the continued support received from the Ontario Ministry of Agriculture Food and Rural Affairs OMAFRA and the Natural Sciences and Engineering Research Council of Canada NSERC
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