Authors: SuIl Kang SeoYoung Kang HorGil Hur
Publish Date: 2008/04/15
Volume: 79, Issue: 4, Pages: 663-
Abstract
Carbamazepine which has been used in the treatments of epilepsy is often found in the environment Although metabolism of carbamazepine by humans and rats has been characterized the environmental fate of carbamazepine has not been studied In this study two model fungi Cunninghamella elegans ATCC 9245 and Umbelopsis ramanniana R56 which have previously shown diverse metabolic activities were tested for metabolism of carbamazepine Both fungi produced three metabolites each C1–C3 and M1–M3 All six metabolites showed M + H+ at m/z 253 suggesting addition of one oxygen to the parent compound Highperformance liquid chromatography and liquid chromatography–mass spectrometric analysis detected 10 11dihydro10 11epoxycarbamazepine as a major product C3 47 and M3 85 and 3hydroxycarbamazepine C2 15 and M2 7 from carbamazepine through mixed monooxidation reactions in both fungal strains C elegans was confirmed to produce 2hydroxycarbamazepine C1 38 while U ramanniana produced a yet unidentified ringhydroxylated metabolite M1 8 The current study suggests that carbamazepine is likely to be subjected to initially diverse monooxygenation reactions by fungal metabolisms resulting in the formation of the corresponding metabolites which were similarly found in mammalian metabolismsThis study was funded by United Nations University and Gwangju Institute of Science and Technology GIST Joint Program on Science and Technology for Sustainability GIST and a grant from the MOST/KOSEF to the Environmental Biotechnology National Core Research Center Grant R152003012020020 Gyoungsang National University Korea
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