Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Appl Microbiol Biotechnol

Search In Journal Title:

Abbravation: Applied Microbiology and Biotechnology

Search In Journal Abbravation:

Publisher

Springer Berlin Heidelberg

Search In Publisher:

DOI

10.1007/s00203-012-0857-3

Search In DOI:

ISSN

1432-0614

Search In ISSN:
Search In Title Of Papers:

Secretory production of singlechain antibody scF

Authors: Masao Tokunaga Makoto Mizukami Koji Yamasaki Hiroko Tokunaga Hiromasa Onishi Hiroshi Hanagata Matsujiro Ishibashi Akira Miyauchi Kouhei Tsumoto Tsutomu Arakawa
Publish Date: 2013/01/20
Volume: 97, Issue: 19, Pages: 8569-8580
PDF Link

Abstract

Halophilic βlactamase BLA has been successfully used as a novel fusion partner for soluble expression of aggregationprone foreign proteins in Escherichia coli cytoplasm Appl Microbiol Biotechnol 86649–658 2010b This halophilic BLA fusion technology was applied here for secretory expression in Brevibacillus The “Brevibacillus in vivo cloning” method recently developed by Higeta Shoyu group for the construction and transformation of Brevibacillus expression vectors facilitates efficient screening of the production conditions of Brevibacillus expression system Two singlechain antibodies scFv HyHEL10 single chain scFv scFvHEL and antifluorescein single chain scFv scFvFLU were successfully secreted to culture supernatant as a fusion protein with halophilic BLA The scFvHELHis purified after cleavage of BLA portion with thrombin was fully active it formed a stoichiometric complex with the antigen lysozyme and inhibited the enzymatic activity The scFvFLUHis similarly expressed and purified stoichiometrically inhibited fluorescence intensity of fluorescein The molecular mass of scFvHELHis was determined to be 27800 Da by light scattering measurements indicating its monomeric structure in solutionWe are grateful to Drs Daisuke Ejima and Haruna Sato for the assistance of SEC MALS measurements This work was supported by a Grant in Aid for Science Research 20580372 23580475 to MT from MEXT Japan and by funding from the Institute for Fermentation Osaka to MT

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Fusion of a family 9 cellulose-binding module improves catalytic potential of Clostridium thermocellum cellodextrin phosphorylase on insoluble cellulose
  2. Heterologous expression of metabotropic glutamate receptor subtype 1 in Saccharomyces cerevisiae
  3. Dynamics of biomass composition and growth during recovery of nitrogen-starved Chromochloris zofingiensis
  4. Biodegradation of sulfamethoxazole by individual and mixed bacteria
  5. Promiscuous activity of ( S , S )-butanediol dehydrogenase is responsible for glycerol production from 1,3-dihydroxyacetone in Corynebacterium glutamicum under oxygen-deprived conditions
  6. Glutathione accumulation in ethanol-stat fed-batch culture of Saccharomyces cerevisiae with a switch to cysteine feeding
  7. Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and microalgae
  8. Enhanced vanillin production from ferulic acid using adsorbent resin
  9. Anaerobic degradation of monoaromatic hydrocarbons
  10. Shifts in microbial communities in bioaugmented grease interceptors removing fat, oil, and grease (FOG)
  11. Stoichiometry of microbial continuous-flow purification of toluene-contaminated air
  12. Community structure and transcript responses of anammox bacteria, AOA, and AOB in mangrove sediment microcosms amended with ammonium and nitrite
  13. Selection of optimal reference genes for expression analysis in the entomopathogenic fungus Beauveria bassiana during development, under changing nutrient conditions, and after exposure to abiotic stresses
  14. Silver-decorated orthorhombic nanotubes of lithium vanadium oxide: an impeder of bacterial growth and biofilm
  15. A thermostable feruloyl-esterase from the hemicellulolytic bacterium Thermobacillus xylanilyticus releases phenolic acids from non-pretreated plant cell walls
  16. Production of protocatechuic acid by Corynebacterium glutamicum expressing chorismate-pyruvate lyase from Escherichia coli
  17. Production and characterisation of recombinant α- l -arabinofuranosidase for production of xylan hydrogels
  18. Identification of novel genes responsible for ethanol and/or thermotolerance by transposon mutagenesis in Saccharomyces cerevisiae
  19. Asymmetric Stetter reactions catalyzed by thiamine diphosphate-dependent enzymes
  20. Molecular identification of the microbial diversity in two sequencing batch reactors with activated sludge
  21. Lytic enzyme discovery through multigenomic sequence analysis in Clostridium perfringens
  22. Perstraction of intracellular pigments by submerged cultivation of Monascus in nonionic surfactant micelle aqueous solution
  23. The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus
  24. Synergistic effect and mechanisms of compound bioflocculant and AlCl 3 salts on enhancing Chlorella regularis harvesting
  25. Understanding the antimicrobial activity behind thin- and thick-rolled copper plates
  26. Safeguarding bacterial resources promotes biotechnological innovation
  27. Characterization of a novel xylanase from Armillaria gemina and its immobilization onto SiO 2 nanoparticles
  28. Safeguarding bacterial resources promotes biotechnological innovation
  29. An exceptionally cold-adapted alpha-amylase from a metagenomic library of a cold and alkaline environment
  30. Impacts of variations in elemental nutrient concentration of Chardonnay musts on Saccharomyces cerevisiae fermentation kinetics and wine composition
  31. Ammonium removal performance of anaerobic ammonium-oxidizing bacteria immobilized in polyethylene glycol gel carrier
  32. Fermentation of quinoa and wheat slurries by Lactobacillus plantarum CRL 778: proteolytic activity
  33. Biosynthesis of ethylene glycol in Escherichia coli
  34. Decolorization of a dye industry effluent by Aspergillus fumigatus XC6
  35. Sugarcane bagasse as support for immobilization of Bacillus pumilus HZ-2 and its use in bioremediation of mesotrione-contaminated soils
  36. A rare sugar xylitol. Part II: biotechnological production and future applications of xylitol
  37. Physiological characterization of recombinant Saccharomyces cerevisiae expressing the Aspergillus nidulans phosphoketolase pathway: validation of activity through 13 C-based metabolic flux analysis
  38. Bacterial quorum sensing and cell surface electrokinetic properties
  39. WS-5995 B, an antifungal agent inducing differential gene expression in the conifer pathogen Heterobasidion annosum but not in Heterobasidion abietinum
  40. Characterization of microbial compositions in a thermophilic chemostat of mixed culture fermentation
  41. Co-occurrence of nitrite-dependent anaerobic ammonium and methane oxidation processes in subtropical acidic forest soils
  42. Fumaric acid production by fermentation
  43. Laser capture microdissection and metagenomic analysis of intact mucosa-associated microbial communities of human colon
  44. The rhizospheres of traditional medicinal plants in Panxi, China, host a diverse selection of actinobacteria with antimicrobial properties
  45. Pilot-scale verification of maximum tolerable hydrodynamic stress for mammalian cell culture
  46. Production of xylanase by an alkaline-tolerant marine-derived Streptomyces viridochromogenes strain and improvement by ribosome engineering
  47. Cloning and expression of a tyrosinase from Aspergillus oryzae in Yarrowia lipolytica : application in l -DOPA biotransformation
  48. Synthesis of flavor and fragrance esters using Candida antarctica lipase
  49. Methane production potential and methanogenic archaea community dynamics along the Spartina alterniflora invasion chronosequence in a coastal salt marsh
  50. Xylose isomerase from polycentric fungus Orpinomyces : gene sequencing, cloning, and expression in Saccharomyces cerevisiae for bioconversion of xylose to ethanol
  51. Nitrogen cycling and relationships between ammonia oxidizers and denitrifiers in a clay-loam soil
  52. Optimization of medium and cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 23F
  53. Biological control of microbial attachment: a promising alternative for mitigating membrane biofouling
  54. The essential role of nitrogen limitation in expression of xplA and degradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) in Gordonia sp. strain KTR9
  55. Engineering of LadA for enhanced hexadecane oxidation using random- and site-directed mutagenesis
  56. Directed evolution of poly[( R )-3-hydroxybutyrate] depolymerase using cell surface display system: functional importance of asparagine at position 285
  57. Identification of fungal metabolites of anticonvulsant drug carbamazepine
  58. Characterization of an exo-acting intracellular α-amylase from the hyperthermophilic bacterium Thermotoga neapolitana
  59. Evaluation of a protective effect of in ovo delivered Campylobacter jejuni OMVs
  60. Construction of a reference plasmid molecule containing eight targets for the detection of genetically modified crops
  61. Biogas desulfurization using autotrophic denitrification process
  62. Novel roles of Bacillus thuringiensis to control plant diseases
  63. Expression of the endogenous and heterologous clavulanic acid cluster in Streptomyces flavogriseus : why a silent cluster is sleeping
  64. Metabolic engineering of gossypol in cotton
  65. Lactulose production by β-galactosidase in permeabilized cells of Kluyveromyces lactis
  66. Assimilable nitrogen utilisation and production of volatile and non-volatile compounds in chemically defined medium by Saccharomyces cerevisiae wine yeasts
  67. Construction of an S-layer protein exhibiting modified self-assembling properties and enhanced metal binding capacities
  68. Conserved and specific responses to hypoxia in Aspergillus oryzae and Aspergillus nidulans determined by comparative transcriptomics
  69. Coupling of enantioselective biooxidation of dl -1,2-propanediol and bioreduction of pinacolone via regeneration cycle of coenzyme
  70. Elimination of diaminopeptidase activity in Pichia pastoris for therapeutic protein production
  71. Characterization of Bacillus spp. strains for use as probiotic additives in pig feed
  72. DNA restriction-modification systems in the ethanologen, Zymomonas mobilis ZM4
  73. Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis and Rhodococcus jostii
  74. Inhibition of anthrax lethal factor: lability of hydroxamate as a chelating group
  75. The transcriptomic profile of Pseudozyma aphidis during production of mannosylerythritol lipids
  76. Effect of red mold rice supplements on serum and meat cholesterol levels of broilers chicken
  77. Enhanced chemical and biological activities of a newly biosynthesized eugenol glycoconjugate, eugenol α - d -glucopyranoside
  78. Production of edible mushrooms in forests: trends in development of a mycosilviculture
  79. A new salicylate synthase AmS is identified for siderophores biosynthesis in Amycolatopsis methanolica 239 T
  80. Effects of the mushroom-volatile 1-octen-3-ol on dry bubble disease
  81. Dehalococcoides abundance and alternate electron acceptor effects on large, flow-through trichloroethene dechlorinating columns
  82. Production of recombinant bacteriocin divercin V41 by high cell density Escherichia coli batch and fed-batch cultures
  83. Carbon fluxes of xylose-consuming Saccharomyces cerevisiae strains are affected differently by NADH and NADPH usage in HMF reduction
  84. Separation of ionic liquid [Mmim][DMP] and glucose from enzymatic hydrolysis mixture of cellulose using alumina column chromatography
  85. Tandem repeat mhBD2 gene enhance the soluble fusion expression of hBD2 in Escherichia coli
  86. A new cytochrome P450 system from Bacillus megaterium DSM319 for the hydroxylation of 11-keto-β-boswellic acid (KBA)
  87. Anti-MRSA and anti-TB metabolites from marine-derived Verrucosispora sp. MS100047
  88. Erratum to: Efficient ( R )-3-hydroxybutyrate production using acetyl CoA-regenerating pathway catalyzed by coenzyme A transferase
  89. Influence of temperature on the effectiveness of a biogenic carbonate surface treatment for limestone conservation
  90. Unraveling the efficient applications of secondary metabolites of various Trichoderma spp.
  91. Anticoagulant heparan sulfate: structural specificity and biosynthesis
  92. Production of indole-3-acetic acid and related indole derivatives from L-tryptophan by Rubrivivax benzoatilyticus JA2
  93. Galacto-oligosaccharide production using microbial β-galactosidase: current state and perspectives
  94. Enhancement of succinate production by metabolically engineered Escherichia coli with co-expression of nicotinic acid phosphoribosyltransferase and pyruvate carboxylase
  95. Enhanced extracellular production of L-asparaginase from Bacillus subtilis 168 by B. subtilis WB600 through a combined strategy
  96. A novel single-chain Fv antibody for connective tissue growth factor against the differentiation of fibroblast into myofibroblast
  97. Strain improvement of Sporolactobacillus inulinus ATCC 15538 for acid tolerance and production of D-lactic acid by genome shuffling
  98. Sugar transporters in efficient utilization of mixed sugar substrates: current knowledge and outlook
  99. Development of a transgenic tobacco plant for phytoremediation of methylmercury pollution
  100. Production of a cytotoxic cationic antibacterial peptide in Escherichia coli using SUMO fusion partner
  101. Bioremediation of trace cobalt from simulated spent decontamination solutions of nuclear power reactors using E . coli expressing NiCoT genes
  102. Current progress in the study on biosynthesis and regulation of ganoderic acids
  103. Electricity production from xylose in fed-batch and continuous-flow microbial fuel cells
  104. An acid and highly thermostable xylanase from Phialophora sp. G5
  105. Flotation as a tool for indirect DNA extraction from soil
  106. An esterase from the basidiomycete Pleurotus sapidus hydrolyzes feruloylated saccharides
  107. Characterization of a ring-hydroxylating dioxygenase from phenanthrene-degrading Sphingomonas sp. strain LH128 able to oxidize benz[ a ]anthracene
  108. Effect of compost, nitrogen salts, and NPK fertilizers on methane oxidation potential at different temperatures
  109. Intracellular expression of Vitreoscilla hemoglobin improves S -adenosylmethionine production in a recombinant Pichia pastoris
  110. Identification, cloning, and characterization of β-glucosidase from Ustilago esculenta
  111. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR
  112. Role of bifidobacteria in the activation of the lignan secoisolariciresinol diglucoside
  113. Enzymatic reduction of complex redox dyes using NADH-dependent reductase from Bacillus subtilis coupled with cofactor regeneration
  114. Construction of a Bacillus thuringiensis engineered strain with high toxicity and broad pesticidal spectrum against coleopteran insects
  115. Evaluation of different Bacillus strains in respect of their ability to produce Surfactin in a model fermentation process with integrated foam fractionation
  116. Improving the thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 using a computationally aided method
  117. Purification and characterization of NAD(P)H-dependent nitroreductase I from Klebsiella sp. C1 and enzymatic transformation of 2,4,6-trinitrotoluene
  118. Achievement of rapid osmotic dehydration at specific temperatures could maintain high Saccharomyces cerevisiae viability
  119. Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols
  120. Demineralization of red crab shell waste by lactic acid fermentation
  121. Production of biologically active recombinant annexin B1 with enhanced stability via a tagging system
  122. A screening system for antioxidants using thioredoxin-deficient yeast: discovery of thermostable antioxidant activity from Agaricus blazei Murill
  123. RETRACTED ARTICLE: Genome sequence and genome mining of a marine-derived antifungal bacterium Streptomyces sp. M10
  124. Structure of bacterial communities in soil following cover crop and organic fertilizer incorporation
  125. Response of activated sludge to the treatment of oxytetracycline production waste stream
  126. Metabolic flux distributions: genetic information, computational predictions, and experimental validation
  127. High production of llama variable heavy-chain antibody fragment (VHH) fused to various reader proteins by Aspergillus oryzae
  128. Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles
  129. Precultivation of Bacillus coagulans DSM2314 in the presence of furfural decreases inhibitory effects of lignocellulosic by-products during l (+)-lactic acid fermentation
  130. Enhancement of free fatty acid production in Saccharomyces cerevisiae by control of fatty acyl-CoA metabolism
  131. Increased Hepatitis B surface antigen production by recombinant Aspergillus niger through the optimization of agitation and dissolved oxygen concentration
  132. Asymmetrical response of anaerobic digestion microbiota to temperature changes
  133. Purification and characterization of arylsulfatase from Sphingomonas sp. AS6330
  134. Greenhouse gas emissions from rice microcosms amended with a plant microbial fuel cell
  135. A cold active (2 R ,3 R )-(−)-di- O -benzoyl-tartrate hydrolyzing esterase from Rhodotorula mucilaginosa
  136. Biodegradation of aromatic compounds: current status and opportunities for biomolecular approaches
  137. Biotechnological aspects of chitinolytic enzymes: a review
  138. Yeast expression platforms
  139. Protein engineering: opportunities and challenges
  140. Uses and production of chiral 3-hydroxy-γ-butyrolactones and structurally related chemicals
  141. Cloning and heterologous expression of plnE , - F , - J and - K genes derived from soil metagenome and purification of active plantaricin peptides
  142. Characterization of the T7 promoter system for expressing penicillin acylase in Escherichia coli
  143. Nitric oxide elicitation for secondary metabolite production in cultured plant cells
  144. Heterologous expression and characterization of a glucose-stimulated β-glucosidase from the termite Neotermes koshunensis in Aspergillus oryzae
  145. Biophotography: concepts, applications and perspectives
  146. Bioconversion of lutein using a microbial mixture—maximizing the production of tobacco aroma compounds by manipulation of culture medium
  147. Production of pigment-free pullulan by swollen cell in Aureobasidium pullulans NG which cell differentiation was affected by pH and nutrition
  148. Overexpression of a type II 3-dehydroquinate dehydratase enhances the biotransformation of quinate to 3-dehydroshikimate in Gluconobacter oxydans
  149. Archaeal DNA polymerases in biotechnology
  150. Mixed consortia in bioprocesses: role of microbial interactions
  151. Improving putrescine production by Corynebacterium glutamicum by fine-tuning ornithine transcarbamoylase activity using a plasmid addiction system
  152. Calmodulin-binding transcription activators and perspectives for applications in biotechnology
  153. Metabolic responses of Rhodococcus erythropolis PR4 grown on diesel oil and various hydrocarbons
  154. Diversity and antibiotic resistance of Acinetobacter spp. in water from the source to the tap
  155. Characterization of biochemical properties and biological activities of biosurfactants produced by Pseudomonas aeruginosa mucoid and non-mucoid strains isolated from hydrocarbon-contaminated soil samples
  156. Expression and surface display of Cellulomonas endoglucanase in the ethanologenic bacterium Zymobacter palmae
  157. Overexpression of Newcastle disease virus (NDV) V protein enhances NDV production kinetics in chicken embryo fibroblasts
  158. Applications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples
  159. Glycerol supplementation of the growth medium enhances in situ detoxification of furfural by Clostridium beijerinckii during butanol fermentation
  160. Bacteria and archaea involved in anaerobic digestion of distillers grains with solubles
  161. Characterization and application of bioflocculant prepared by Rhodococcus erythropolis using sludge and livestock wastewater as cheap culture media
  162. Studies on the production of conjugated linoleic acid from linoleic and vaccenic acids by mixed rumen protozoa
  163. Characterization and engineering of the ethylmalonyl-CoA pathway towards the improved heterologous production of polyketides in Streptomyces venezuelae
  164. Molecular understanding of aminoglycoside action and resistance
Search Result: