Authors: E Bayrak Tabar F Yurt Lambrecht C Gunduz M Yucebas
Publish Date: 2011/02/11
Volume: 288, Issue: 3, Pages: 839-844
Abstract
Radiolabeled molecules have an important role to evaluate tumor characteristics such as aggressiveness and to identify the effectiveness of cancer treatments such as chemotherapy and radiotherapy Various radionuclide 18F 99mTc 124I labeled molecules can be used apoptosis detection by estimating decrescendos cell viability after therapy 99mTctetrofosmin which is used as a myocardial perfusion imaging agent in routine and at the same time is known to accumulate in various tumors including breast tumor The aim of this study was to assess the utility of 99mTctetrofosmin for monitoring the early response of MCF7 breast cancer to chemotherapy To evaluate the role of 99mTctetrofosmin in vitro chemotherapy the uptake ratio was determined using MCF7 breast cancer line after the cells had been treated with cisplatin When we examined the apoptotic ratios which induced with different dose of cisplatin in MCF7 breast cancer cells by using Annexin V and TUNEL methods it was observed that the rate of apoptosis increased with soaring dose The uptake rates of 99mTctetrofosmin in MCF7 cell line in the chemotherapeutic groups were lower than it is in the control group p 001 The negative correlation between uptake ratios and apoptotic rates shows that 99mTctetrofosmin may be used a radiopharmaceutical for evaluating chemotherapy response 99mTctetrofosmin might be probably useful as an imaging agent for estimation of early chemotherapy response in breast cancer
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