Authors: Hamidreza Zolata Hossein Afarideh Fereydoun AbbasiDavani
Publish Date: 2014/03/21
Volume: 301, Issue: 2, Pages: 451-460
Abstract
In this research we develop dual modality molecular imaging and also radioimmunotherapy RIT bioprobes in the form of modified superparamagnetic iron oxide nanoparticles SPIONs conjugated to radiolabeled antibodies for PET and MRI of HER2 expressing cancers as well as a PH sensitive drug carrier by embedded an anticancer agent for cancer therapeutic applications The bioprobes were developed by conjugating 64Cu labeled trastuzumab herceptin and rituximab Anti CD20 antibodies to modified SPIONs The SPIONs were modified with carboxymethyl chitosan and functionalized with acrylic acid AA Also with the purpose of identifying more effective bifunctional chelator BFC we compared the properties of novel BFC pNO2BnPCTA with the commonly used DOTANHS for radioimmunoconjugate preparations Moreover a chemotherapy drug doxorubicin was then loaded onto engineered nanoparticles for targeted intracellular drug delivery and selective cancer cell killing Resulting radioimmunoconjugatedSPIONs were evaluated for molecular imaging and effective targeting of the HER2+ receptors in SKBR3 cell lines and breast tumor bearing Balb/C mice Therefore our biocompatible SPIONs could serve as a promising multifunctional theranostics nanoplatform in dual modality imaging guided RIT of HER2 overexpressing cancer applicable to drug delivery and controlled drug release for trigger both intrinsic and extrinsic pathways of apoptosis
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