Authors: Madhavi S Revankar Kiran M Desai S S Lele
Publish Date: 2007/05/11
Volume: 143, Issue: 1, Pages: 16-26
Abstract
Laccase production by solidstate fermentation SSF using an indigenously isolated white rot basidiomycete Ganoderma sp was studied Among the various agricultural wastes tested wheat bran was found to be the best substrate for laccase production Solidstate fermentation parameters such as optimum substrate initial moisture content and inoculum size were optimized using the onefactoratatime method A maximum laccase yield of 2400 U/g dry substrate U/gds was obtained using wheat bran as substrate with 70 initial moisture content at 25°C and the seven agar plugs as the inoculum Further enhancement in laccase production was achieved by supplementing the solidstate medium with additional carbon and nitrogen source such as starch and yeast extract This medium was optimized by response surface methodology and a fourfold increase in laccase activity 10050 U/g dry substrate was achieved Thus the indigenous isolate seems to be a potential laccase producer using SSF The process also promises economic utilization and value addition of agroresidues
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