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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer-Verlag

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DOI

10.1007/s11403-006-0004-9

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1559-0291

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A Study on the Stability and Enzymatic Activity of

Authors: Stefania Pucciarelli Giulia Bonacucina Franco Bernabucci Marco Cespi Giovanna Mencarelli Giusi Serena De Fronzo Paolo Natalini Giovanni Filippo Palmieri
Publish Date: 2012/04/28
Volume: 167, Issue: 2, Pages: 298-313
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Abstract

Yeast alcohol dehydrogenase ADH is an enzyme widely studied for biotechnological applications due to its involvement in fermentation industry and various attempts to improve its catalytic properties and its thermal stability have been carried out In this paper the influence of a block copolymer Poloxamer 407 on ADH enzymatic activity and thermal behaviour has been studied in order to get new insights about the use of poloxamers in formulation of sustained release systems for therapeutic proteins Poloxamer 407 has the ability to form micelles and gel due to its selfassembling and thermoresponsive properties The effect of the copolymer towards thermal stress and pH changes which often reduce enzymes activity it has been investigated by means of enzymatic assays and differential scanning calorimetry Results showed that at pH 91 and 73 the Poloxamer in the form of unimeric micellar and gel state is able to effectively preserve the enzyme from thermoinactivation In addition by calorimetric data Poloxamer 407 has showed an effect in preserving ADH from aggregation at pH 73 In conclusion Poloxamer 407 seems to be very effective in protecting ADH from stress related events like alkaline inactivation and aggregation

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