Authors: Md Mahabubur Rahman Talukder Simon Choo Sze Shiong
Publish Date: 2015/09/15
Volume: 177, Issue: 8, Pages: 1742-1752
Abstract
Although ultrasound has been used to accelerate many enzymatic reactions the low stability of enzymes in such a system still remains a critical issue limiting its industrial application Here we have reported that polyethylene glycol PEG pretreatment stabilized Chromobacterium viscosum lipase CVL in ultrasoundassisted waterisooctane emulsion PEGs of different molecular weights and concentrations were used to pretreat CVL and the pretreated lipase activities for olive oil hydrolysis were investigated at different ultrasonic powers The best result was attained with PEG400 at 100 mg/ml for a lipase concentration of 002 mg/ml and an ultrasonic power of 106 W The halflife time of PEG400treated lipase at 106 W was 54 min a 27fold higher than that attained using untreated lipase Circular dichroism CD spectra suggested that PEG increased the rigidity of CVL structure which favored the lipase stability against ultrasound inactivation These results have important implications for the exploitation of ultrasound in biocatalytic process
Keywords: