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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer US

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DOI

10.1007/bf02089715

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ISSN

1559-0291

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Isolation and Immobilization of Influenza VirusSp

Authors: Susana Araceli Carrizales Álvarez Anna Ilyina Karla Monzerratt Gregorio Jáuregui José Luis Martínez Hernández Brenda Berenice Vazquez Gutiérrez Elda Patricia Segura Ceniceros Alejandro Zugasti Cruz Hened Saade Caballero Raúl Guillermo López Campos
Publish Date: 2014/08/27
Volume: 174, Issue: 5, Pages: 1945-1958
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Abstract

Avian influenza viruses preferentially bind to sialic acid alpha23galactose NSAα23Gal receptors on epithelial cells Herein we describe a procedure we have developed for isolation of NSAα23Gal receptors from porcine trachea using magnetic nanoparticles NPs coated with chitosan NPCh and functionalized with Maackia amurensis lectin NPlectin Magnetic nanoparticles were coated with chitosan in a onestep coprecipitation and then M amurensis lectin was immobilized covalently using glutaraldehyde Lectin coated nanoparticles were incubated with sialic acid enriched fraction of tracheal homogenate and NSAα23Gal receptor was extracted under magnetic field in two cycles The presence of 664 kDa protein was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis SDSPAGE The interaction of immobilized receptor NPChR with M amurensis lectin NPChRL was demonstrated by Fourier transform infrared spectrometry FTIR and thermogravimetric analysis TGA

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