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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer US

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DOI

10.1016/j.biocon.2004.06.017

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1559-0291

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Artificial Enzyme Mimics for Catalysis and Double

Authors: Xiaohua Li Zhujun Zhang Yongbo Li
Publish Date: 2013/11/29
Volume: 172, Issue: 4, Pages: 1859-1865
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Abstract

This work presents a new chemiluminescent CL probe array assay The new type CL probe array is based on enzyme mimics of Co3O4–SiO2 mesoporous nanocomposite material which not only have an excellent catalytic effect on the luminol–H2O2 CL reaction in an alkaline medium but also can be used for the immobilization of enzymes The linear range of the lactose concentration is 30 × 10−7 to 10 × 10−5 g mL−1 and the detection limit is 69 × 10−8 g mL−1 βGalactosidase and glucose oxidase were selected as a model for enzyme assays to demonstrate the applicability of Co3O4–SiO2 mesoporous nanocomposite material in multienzyme immobilization The novel bifunctional CL probe array has been successfully applied to the determination of lactose in milk

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