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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Humana Press Inc

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1559-0291

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A Thermostable Alkaline Lipase from a Local Isolat

Authors: Eltayib Hassan Ahmed Tripti Raghavendra Datta Madamwar
Publish Date: 2009/08/23
Volume: 160, Issue: 7, Pages: 2102-2113
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Abstract

A mesophilic bacterial culture producing a novel thermostable alkaline lipase was isolated from oil rich soil sample and identified as Bacillus subtilis EH 37 The lipase was partially purified by ammonium sulfate precipitation and hydrophobic interaction chromatography with 178fold purification and 419 U/ml specific activity The partially purified enzyme exhibited maximum activity at pH 80 and at 60 °C It retained 100 of activity at 50 °C and 60 °C for 60 min The presence of Ca+2 Mg+2 and Zn2+ exhibited stimulatory effect on lipase activity whereas Fe+3 and Co+2 reduced its activity The enzyme retained more than 80 of its initial activity upon exposure to organic solvents exhibited 107 and 115 activity in the presence of 15 isopropyl alcohol and 30 nhexane respectively The EH 37 lipase also proved to be an efficient catalyst in synthesis of ethyl caprylate in organic solvent thus providing a concept of application of B subtilis lipase in nonaqueous catalysis

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