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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer-Verlag

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DOI

10.1002/gps.2751

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1559-0291

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Improvement in the Purification Process of the Cap

Authors: Silvia Maria Ferreira Albani Mateus Ribeiro da Silva Mickie Takagi Joaquin CabreraCrespo
Publish Date: 2012/06/05
Volume: 167, Issue: 7, Pages: 2068-2075
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Abstract

Capsular polysaccharide produced by Haemophilus influenzae b Hib is the main virulent agent and used as the antigen in the vaccine formulation In this study an improved process of polysaccharide purification was established based on tangential flow ultrafiltration using detergents cocamidopropyl betaine and sodium deoxycholate two selective ethanol precipitations steps and extensive enzymatic hydrolysis as strategy The relative purity RP related to protein and nucleic acids were 122∼263 and 294∼480 respectively and compatible with the specifications established by the World Health Organization for Hib vaccine RP ≥ 100 These results make this process simple cheaper efficient environmentally friendly and prone to be scaled upWe gratefully acknowledge the financial support from the São Paulo Research Foundation FAPESP 2007/500822 and the Butantan Foundation—Brazil Silvia MF Albani received a scholarship from the Secretary of Education of São Paulo State We also thank Mr Lourivaldo Inácio de Souza and Ms Inês do Amaral Maurelli for the technical assistance

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