Journal Title
Title of Journal: Appl Biochem Biotechnol
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Abbravation: Applied Biochemistry and Biotechnology
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Publisher
Springer-Verlag
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Authors: Zhaolei Liu Chunsun Gu Fadi Chen Jiafu Jiang Yinghao Yang Peiling Li Sumei Chen Zhen Zhang
Publish Date: 2012/07/22
Volume: 168, Issue: 2, Pages: 383-391
Abstract
Arabidopsis transcription factor APETALA2 AP2 controls multiple aspects of plant growth and development including seed development stem cell maintenance and specification of floral organ identity Based on sequence similar of Arabidopsis AP2 and its homologues genes from other plant species degenerate RTPCR and rapid amplification of cDNA ends assay were used to clone AP2 genes from lotus Nelumbo nucifera A 2048bp cDNA fragment was obtained which contains a 1536bp open reading frame encoding a protein of 511 amino acids The protein contains two AP2 domains that are conserved in AP2 proteins from other plant species thus was named as N nucifera APETALA2 NnAP2 Quantitative RTPCR revealed that NnAP2 gene was expressed in flowers roots leaves and stems of N nucifera with flowers which have the highest transcript levels Further analysis showed that in all five lotus cultivars examined including “Zhongguogudailian” “Yaoniangyujiao” “Jinxia” “Hongtailian” and “Yiliangqianban” petals always have the highest expression levels when compared with the other four flower organs though the number of petals in these cultivars ranged from simple to thousands However NnAP2 expression level in four nonsimple petal flower cultivars was higher than that in the simple petal flower cultivar Zhongguogudailian indicating that NnAP2 may play a role in specification of petal identity during the evolutionary process of the ancient species N nuciferaThis study was supported by the National Natural Science Foundation of China grant numbers 30872064 31071820 and 31071825 the Program for HiTech Research Jiangsu China grant numbers BE2008307 BE2009317 and BE2010303 and the Fundamental Research Funds for the Central Universities KYJ 200907
Keywords:
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