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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Humana Press Inc

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DOI

10.1007/978-1-4020-9237-4_19

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ISSN

1559-0291

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Simulated Microgravity Affects Growth of Emphasis

Authors: Liang Xiang Feng Qi DaZhang Dai Chun Li YuanDa Jiang
Publish Date: 2009/11/18
Volume: 162, Issue: 3, Pages: 654-661
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Abstract

Effects of simulated microgravity SMG on bacteria have been studied in various aspects However few reports are available about production of recombinant protein expressed by bacteria in SMG In this study growth of E coli BL21 DE3 cells transformed with pET28a +pgus in doubleaxis clinostat that could model low shear SMG environment and the recombinant βdglucuronidase PGUS expression have been investigated Results showed that the cell dry weights in SMG were 1647 3806 and 2879 more than normal gravity NG control and the efficiency of the recombinant PGUS expression in SMG were 1833 1936 and 3342 higher than that in NG at 19 °C 28 °C and 37 °C respectively P  005

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  2. Solid-state Fermentation for Enhanced Production of Laccase using Indigenously Isolated Ganoderma sp.
  3. Production of Thermostable Lipase by Thermomyces lanuginosus on Solid-State Fermentation: Selective Hydrolysis of Sardine Oil
  4. Expression, Purification, and Characterization of Endo-β- N -Acetylglucosaminidase H Using Baculovirus-Mediated Silkworm Protein Expression System
  5. Exhausted Jackknife Validation Exemplified by Prediction of Temperature Optimum in Enzymatic Reaction of Cellulases
  6. Intrinsic Characteristics of Cr 6+ -Resistant Bacteria Isolated from an Electroplating Industry Polluted Soils for Plant Growth-Promoting Activities
  7. Phytase Production and Development of an Ideal Dephytinization Process for Amelioration of Food Nutrition Using Microbial Phytases
  8. Cunninghamella as a Microbiological Model for Metabolism of Histamine H 3 Receptor Antagonist 1-[3-(4- tert -Butylphenoxy)propyl]piperidine
  9. Synthesis, Characterization, and Oil Recovery Application of Biosurfactant Produced by Indigenous Pseudomonas aeruginosa WJ-1 Using Waste Vegetable Oils
  10. Regeneration-Based Quantification of Coumarins (Scopoletin and Scoparone) in Abutilon indicum In Vitro Cultures
  11. Identification of New Dominant-Negative Mutants of Anthrax Protective Antigen Using Directed Evolution
  12. Galanthamine and Related Alkaloids Production by Leucojum aestivum L. Shoot Culture using a Temporary Immersion Technology
  13. A Study on the Stability and Enzymatic Activity of Yeast Alcohol Dehydrogenase in Presence of the Self-Assembling Block Copolymer Poloxamer 407
  14. Lignin Peroxidase from Streptomyces viridosporus T7A: Enzyme Concentration Using Ultrafiltration
  15. Identification and Expression of an APETALA2 -Like Gene from Nelumbo nucifera
  16. Identification and Expression of an APETALA2 -Like Gene from Nelumbo nucifera
  17. Loops Adjacent to Catalytic Region and Molecular Stability of Man1312
  18. Optimisation of Batch Culture Conditions for Cell-Envelope-Associated Proteinase Production from Lactobacillus delbrueckii subsp. lactis ATCC® 7830™
  19. Prokaryotic Expression, Purification and Characterization of Aspergillus sulphureus β-Mannanase and Site-Directed Mutagenesis of the Catalytic Residues
  20. The Bioconversion of Pretreated Cashew Apple Bagasse into Ethanol by SHF and SSF Processes
  21. Biosorption of Heavy Metal Ions (Cu 2+ , Mn 2+ , Zn 2+ , and Fe 3+ ) from Aqueous Solutions Using Activated Sludge: Comparison of Aerobic Activated Sludge with Anaerobic Activated Sludge
  22. Improvement of Regio-Specific Production of Myricetin-3- O -α- l -Rhamnoside in Engineered Escherichia coli
  23. Purification of Alcohol Dehydrogenase from Saccharomyces cerevisiae Using Magnetic Dye-Ligand Affinity Nanostructures
  24. A Novel Hatching Enzyme from Starfish Asterias amurensis : Purification, Characterization, and Cleavage Specificity
  25. Purification, Sequencing, and Biochemical Characterization of a Novel Calcium-Independent α-Amylase AmyTVE from Thermoactinomyces vulgaris
  26. Purification, Sequencing, and Biochemical Characterization of a Novel Calcium-Independent α-Amylase AmyTVE from Thermoactinomyces vulgaris
  27. Improvement in the Purification Process of the Capsular Polysaccharide from Haemophilus influenzae Type b by Using Tangential Ultrafiltration and Diafiltration
  28. A Thermostable Alkaline Lipase from a Local Isolate Bacillus subtilis EH 37: Characterization, Partial Purification, and Application in Organic Synthesis
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  39. Antifungal Activity of Diketopiperazines and Stilbenes Against Plant Pathogenic Fungi In Vitro
  40. Isolation, Purification and Characterisation of an Organic Solvent-Tolerant Ca 2+ -Dependent Protease from Bacillus megaterium AU02
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  48. Enzymatic Hydrolysis of Polylactic Acid Fiber
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  50. Kinetic Studies on the Product Inhibition of Enzymatic Lignocellulose Hydrolysis
  51. Partial Characterization of the Proteolytic Properties of an Enzymatic Extract From “Aguama” Bromelia pinguin L. Fruit Grown in Mexico
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