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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer US

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DOI

10.1016/0167-9457(94)90046-9

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1559-0291

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Synthesis Characterization and DNA Binding Studi

Authors: Nahid Shahabadi Saba Hadidi Avat Arman Taherpour
Publish Date: 2014/01/07
Volume: 172, Issue: 5, Pages: 2436-2454
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Abstract

The watersoluble platinumII complex PtLVdmsoClCl LV is Levetiracetam an antiepileptic drug belonging to the pyrrolidine family was synthesized and characterized using physicochemical spectroscopic and computational methods Multispectroscopic methods used to study the interaction of PtII complex with calf thymus ctDNA in Tris–HCl buffer solution pH 74 The PtII complex was found to be a minor groove binder with a preference for the AT region compared to the GC region This was substantiated by displacement studies with Hoechst 33258 a known minor groove binder The mean binding constant obtained from the spectroscopic techniques was 7 × 104 M−1 In comparison with the previous study the binding constant of PtII complex is 15 times greater than the corresponding value of levetiracetam These results indicate that the binding affinity of PtII complex is stronger than levetiracetam

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