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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer-Verlag

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DOI

10.1002/ecja.4410700912

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1559-0291

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Synthesis of Pure Emphasis Type="Italic"meso/Em

Authors: Soojin Lee Borim Kim Kyungmoon Park Youngsoon Um Jinwon Lee
Publish Date: 2012/03/21
Volume: 166, Issue: 7, Pages: 1801-1813
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Abstract

meso23Butanediol meso23BDO is essential for the synthesis of various economically valuable biosynthetic products however the production of meso23BDO from expensive carbon sources is an obstacle for industrial applications In this study genes involved in the synthesis of 23BDO in Klebsiella pneumoniae were identified and used to genetically modify Escherichia coli for meso23BDO production Two 23BDO biosynthesis genes—budA encoding acetolactate and mesobudC encoding mesoSADH—from K pneumoniae were cloned into the pUC18 plasmid and introduced into E coli In 2 l batch culture the SGSB03 E coli strain yielded meso23BDO at 031 g/gglucose with a maximum of 157 g/lculture after 48 h and 021 g/gcrude glycerol with a maximum of 69 g/lculture after 48 h Batch cultures were grown under optimized conditions aerobic 6 carbon source 37 °C and initial pH 7 To find the optimal culture conditions for meso23BDO production we evaluated the enzyme activity of mesoSADH and the whole cell conversion yield meso23BDO/acetoin of the E coli SGSB02 which contains pSB02 mesoSADH showed high enzyme activity at 30–37 °C and pH 7 305–415 U/mg of protein and the conversion yield of SGSB02 E coli was highest at 37–42 °C and a pH of 7 025–028 g meso23BDO/gacetoinThis research was supported by the RD Program of Ministry of Knowledge Economy MKE/KEIT no 10035578 Development of 23BDO and derivative production technology for CZero bioplatform industry This work was supported by the Graduate School of Specialization for Biotechnology Program of the MKE

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