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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer-Verlag

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DOI

10.1016/0013-7944(89)90208-7

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1559-0291

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Optimisation of Batch Culture Conditions for Cell

Authors: Dominic Agyei Ravichandra Potumarthi Michael K Danquah
Publish Date: 2012/08/16
Volume: 168, Issue: 5, Pages: 1035-1050
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Abstract

Using a combination of conventional sequential techniques the batch growth conditions for the production of cellenvelopeassociated proteinases have for the first time been studied and optimised in Lactobacillus delbrueckii subsp lactis 313 ATCC 7830 LDL 313 Concentrations of inoculum 01  X  10  vol/vol agitation speed 0  S  200 rpm varying incubation temperature 30  T  50 °C starting pH 45  pH  7 and carbon/nitrogen ratio of production medium 02  r  5 had an individual effect on proteinase yield p  001 Optimal conditions for proteinase production included an initial pH of 60 45 °C incubation temperature 2  v/v inoculum size of OD560 = 1 150 rpm agitation speed and growth medium carbon/nitrogen ratio of 10 Maximum proteinase activity obtained for whole cells was 099 U/ml after 8 h of incubation The variables studied are very relevant due to their significance in improving the productivity of proteinase synthesis from LDL 313 under process and likely economic optimum conditions

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