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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer-Verlag

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DOI

10.1016/0167-6687(93)90855-j

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1559-0291

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Purification of Alcohol Dehydrogenase from Emphas

Authors: Nazife Kaya Deniz Aktaş Uygun Sinan Akgöl Adil Denizli
Publish Date: 2013/02/14
Volume: 169, Issue: 7, Pages: 2153-2164
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Abstract

Reactive Green 19 was covalently immobilized onto magnetic nanostructures for purification of alcohol dehydrogenase from Saccharomyces cerevisiae The Reactive Green 19 immobilized magnetic nanostructures were characterized by Fourier transform infrared spectroscopy electron spin resonance atomic force microscope and energy dispersive Xray analysis Particle size of nanostructures was found to be roughly 70 nm Alcohol dehydrogenase adsorption experiments were investigated under different conditions in batch system ie medium pH alcohol dehydrogenase concentration temperature and ionic strength Maximum alcohol dehydrogenase adsorption capacity was found to be 17609 mg/g polymer while nonspecific alcohol dehydrogenase adsorption onto plain magnetic nanostructures was negligible 194 mg/g polymer Alcohol dehydrogenase molecules were desorbed by using 10 M NaCl with 984  recovery Alcohol dehydrogenase from S cerevisiae was purified 4563fold in single step with dyeimmobilized magnetic nanostructures and purity of alcohol dehydrogenase was shown by silverstained sodium dodecyl sulfatepolyacrylamide gel electrophoresis

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