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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Humana Press Inc

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DOI

10.1002/aja.1000400203

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ISSN

1559-0291

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New Analytical Method using Coupled Enzymes for De

Authors: Ines Ben Rejeb Lotfi Monser Mohamed Gargouri
Publish Date: 2010/03/01
Volume: 162, Issue: 6, Pages: 1536-1546
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Abstract

A new simple and original method is described for specific measurement of polyunsaturated fatty acid content in olive oil This analytical system uses coupled enzymes lipase and lipoxygenase The system consists of lipasecatalyzed hydrolysis of triacylglycerol and subsequent lipoxygenation of liberated polyunsaturated fatty acids The hydroperoxyfatty acids formed were easily monitored by spectrophotometry at 234 nm After being optimized the method was validated in terms of linearity precision sensitivity and recovery Linear calibration graph was obtained in the range 50–500 µg mL−1 with a correlation coefficient higher than 0921 and a detection limit S/N = 3 of 15 µg mL−1 The precision of the method relative standard deviation for within and between days is better than 7 and 12 respectively The proposed method was successfully applied to the estimation of polyunsaturated fatty acids level in olive oil samples and results obtained were in excellent agreement with those obtained by the classical official method The proposed method is accurate simple cheap and can be satisfactorily used for routine analysis of edible oils

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  2. Solid-state Fermentation for Enhanced Production of Laccase using Indigenously Isolated Ganoderma sp.
  3. Production of Thermostable Lipase by Thermomyces lanuginosus on Solid-State Fermentation: Selective Hydrolysis of Sardine Oil
  4. Expression, Purification, and Characterization of Endo-β- N -Acetylglucosaminidase H Using Baculovirus-Mediated Silkworm Protein Expression System
  5. Exhausted Jackknife Validation Exemplified by Prediction of Temperature Optimum in Enzymatic Reaction of Cellulases
  6. Intrinsic Characteristics of Cr 6+ -Resistant Bacteria Isolated from an Electroplating Industry Polluted Soils for Plant Growth-Promoting Activities
  7. Phytase Production and Development of an Ideal Dephytinization Process for Amelioration of Food Nutrition Using Microbial Phytases
  8. Cunninghamella as a Microbiological Model for Metabolism of Histamine H 3 Receptor Antagonist 1-[3-(4- tert -Butylphenoxy)propyl]piperidine
  9. Synthesis, Characterization, and Oil Recovery Application of Biosurfactant Produced by Indigenous Pseudomonas aeruginosa WJ-1 Using Waste Vegetable Oils
  10. Regeneration-Based Quantification of Coumarins (Scopoletin and Scoparone) in Abutilon indicum In Vitro Cultures
  11. Identification of New Dominant-Negative Mutants of Anthrax Protective Antigen Using Directed Evolution
  12. Galanthamine and Related Alkaloids Production by Leucojum aestivum L. Shoot Culture using a Temporary Immersion Technology
  13. A Study on the Stability and Enzymatic Activity of Yeast Alcohol Dehydrogenase in Presence of the Self-Assembling Block Copolymer Poloxamer 407
  14. Lignin Peroxidase from Streptomyces viridosporus T7A: Enzyme Concentration Using Ultrafiltration
  15. Identification and Expression of an APETALA2 -Like Gene from Nelumbo nucifera
  16. Identification and Expression of an APETALA2 -Like Gene from Nelumbo nucifera
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  18. Optimisation of Batch Culture Conditions for Cell-Envelope-Associated Proteinase Production from Lactobacillus delbrueckii subsp. lactis ATCC® 7830™
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  20. The Bioconversion of Pretreated Cashew Apple Bagasse into Ethanol by SHF and SSF Processes
  21. Biosorption of Heavy Metal Ions (Cu 2+ , Mn 2+ , Zn 2+ , and Fe 3+ ) from Aqueous Solutions Using Activated Sludge: Comparison of Aerobic Activated Sludge with Anaerobic Activated Sludge
  22. Improvement of Regio-Specific Production of Myricetin-3- O -α- l -Rhamnoside in Engineered Escherichia coli
  23. Purification of Alcohol Dehydrogenase from Saccharomyces cerevisiae Using Magnetic Dye-Ligand Affinity Nanostructures
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  39. Antifungal Activity of Diketopiperazines and Stilbenes Against Plant Pathogenic Fungi In Vitro
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