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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer-Verlag

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DOI

10.1016/0921-4534(94)92410-4

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1559-0291

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Heterologous Expression and Characterization of a

Authors: Masato Otagiri Crisanto M Lopez Katsuhiko Kitamoto Manabu Arioka Toshiaki Kudo Shigeharu Moriya
Publish Date: 2013/01/25
Volume: 169, Issue: 6, Pages: 1910-1918
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Abstract

The termite symbiotic system is one of the efficient lignocellulose degradation systems We tried to express and characterize a novel cellulolytic enzyme from this system Here we report the isolation of an endoβ14glucanase gene homolog of glycoside hydrolase family 45 from a symbiotic protistan community of Reticulitermes speratus Heterologous expression of this gene was performed using the expression system of Aspergillus oryzae Analysis of enzymatic properties revealed 786 μmol/min/mg protein in specific activity a V max of 8330 units/mg protein and a K m value of 258 mg/ml with carboxymethyl cellulose as the substrate Thinlayer chromatography analysis showed that RsSymEG2 produces cellobiose from cellodextrins larger than cellohexaose This enzyme showed high specific activity like other endoβ14glucanases from the symbiotic system of termites It means that the termite symbiotic system is a good resource for highly active endoβ14glucanasesThis research was supported by the Program for the Promotion of Basic Research Activity for Innovative Biosciences PROBRAIN Partial support was also provided by the Biomass Engineering Research Program RIKEN CML was supported by the UNESCO Postgraduate Interuniversity Course in Biotechnology

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